Stevia (Stеvia rebaudiana B.) as a medicinal plant and its antioxidant and lipid-modulating effects with focus on ruminant production. A review
Pencho Penchev
, Stanimir Enchev
, Radena Nenova
, Yordanka Ilieva
, Desislava Ivanova, Matthias Schreiner
Abstract: A comprehensive literature search was conducted, and the expertise and achievements with stevia and some other medicinal plants of our plant and animal breedeing teams were taken in consideration. The objective to summarize the background of the medicinal applications of stevia was generally fuilfilled, the found available sources treating the effects on ruminant products is limited. Besides its direct benefits to consumers, indirect effect of its glycosides should be especially sought on production of ruminant origin, which is notorious for its saturated lipids. Except its immunomodulatory, anti-inflammatory, anti-oxidative and anti-tumor properties, this review implies stevia’s capacity to modulate the anti-oxidant and nutritional qualities of foodstuffs. Not many proofs have been found for the influence of stevia on fatty acid composition of ruminant products, but its effects on rumen processes suggest application as a natural modulator of lipid quality via manipulation of rumen environment and functions. All this necessiates further in-depth research, but to date the available scientific evidence affords the presumption that it is worthwile to grow stevia for such purposes, justifying the peculiarities of its cultivation (vegetatively bred and maintained in vitro) and the efforts in the latitudes of Bulgaria, where it needs to be treated like an annual, not like a perennial plant as in its continent of origin.
Keywords: buffalo; fatty acids; medicinal plants; stevia, antioxidant
Citation: Penchev, P., Enchev, S., Nenova, R., Ilieva, Y., Ivanova, D. & Schreiner, M. (2025). Stevia (Stеvia rebaudiana B.) as a medicinal plant and its antioxidant and lipid-modulating effects – with focus on ruminant production. A review. Bulg. J. Agric. Sci., 31(3), 595–604.
| References: (click to open/close) | Abou-Arab, A. E., Abou-Arab, A. A. & Abu-Salem, M. F. (2010). Physico-chemical assessment of natural sweeteners steviosides produced from Stevia rebaudiana Bertoni plant. African Journal of Food Science, 4-5, 269–281.
Aghajanyan, A., Movsisyan, Z. & Trchounian, A. (2017). Antihyperglycemic and antihyperlipidemic activity of hydroponic Stevia rebaudiana aqueous extract in hyperglycemia induced by immobilization stress in rabbits. BioMed. Research International, 2017(1), 9251358. DOI:10.1155/2017/9251358.
Ahmad, N., Fazal, H., Abbasi, B. & Farooq, S. (2010). Efficient free radical scavenging activity of Ginkgo biloba, Stevia rebaudiana and Parthenium hysterophorous leaves through DPPH (2,2-diphe-nyl-1-picrylhydrazyl). International Journal of Phytomedicine, 2(3), 231–239.
Ahmad, J., Khan, I., Blundell, R., Azzopardi, J. & Mahomoodally, M. F. (2020). Stevia rebaudiana Bertoni: an updated review of its health benefits, industrial applications and safety. Trends in Food Science & Technology, 100, 177–189.
Akihisa, T., Hamasaki, Y., Tokuda, H., Ukiya, M., Kimura, Y. & Nishino, H. (2004). Microbial transformation of isosteviol and inhibitory effects on Epstein-Barr virus activation of the transformation products. Journal of Natural Products, 67(3), 407–410.
Allam, S. M., El Hosseiny, H. M., Gawad, A. M. A., El-Saadany, S. A. & Zeid, A. M. M. (1999). Medicinal herbs and plants as feed additives for ruminant. I. Effect of using some medicinal herbs and plants as feed additives on Zaraibi goat performance. Egyptian Journal of Nutrition and Feeds, 2(Special Issue), 349–365.
Ashes, J. R., Welch, P. S. V., Gulati, S. K., Scott, T. W. & Brown, G. H. (1992). Manipulation of the fatty acid composition of milk by feeding protected canola seeds. Journal of Daily Science, 75(4), 1090–1096.
Atteh, J. O., Onagbesan, O. M., Tona, K., Decuypere, E., Geuns, J. M. & Buyse, J. (2008). Evaluation of supplementary stevia (Stevia rebaudiana Bertoni) leaves and stevioside in broiler diets: effects on feed intake, nutrient metabolism, blood parameters and growth performance. Journal of Animal Physiology and Animal Nutrition, 92(6), 640–649.
Atteh, J. O., Onagbesan, O. M., Tona, K., Buyse, J., Decuypere, E. & Geuns, J. M. (2011). Potential use of Stevia rebaudiana in animal feeds. Archivos de Zootecnia, 60(229), 133–136.
Bauman, D. E., Mather, I. H., Wall, R. J. & Lock, A. L. (2006). Major advances associated with the biosynthesis of milk. Journal of Dairy Science, 89(4), 1235–1243.
Belury, M. A. (2002). Dietary conjugated linoleic acid in health: Physiological effects and mechanisms of action. Annual Review of Nutrition, 22(1), 505–531.
Bender, C., Graziano, S. & Zimmermann, B. F. (2015). Study of Stevia rebaudiana Bertoni antioxidant activities and cellular properties. International Journal of Food Sciences and Nutrition, 66(5), 553–558.
Benford, D. J., DiNovi, M. & Schlatter, J. (2006). Safety evaluation of certain food additives: Steviol Glycosides” (PDF – 18MB). WHO Food Additives Series (World Health Organization Joint FAO/WHO Expert Committee on Food Additives (JECFA) 54.
Beyero, N., Kapoor, V. & Tewatia, B. S. (2015). Effect of different roughage: Concentrate ratio on milk yield and its fatty acid profile in dairy cows. Journal of Biology, Agriculture and Healthcare, 5(13), 176–185.
Brandie, J. (2004). FAQ – Stevia, nature, s natural low calorie sweetener. Agriculture and Agri-Food, Canada. http://res2.agr.ca/London/faq/ stevia_e.htm.
Carakostas, M., Curry, L., Boileau, A. & Brusick, D. (2008). Overview: The history, technical function and safety of rebaudioside A, a naturally occurring steviol glycoside, for use in food and beverages. Food and Chemical Toxicology, 46(7), S1–S10 DOI: 10.1016/j.fct.2008.05.003.
Cedillo, J., Kholif, A. E., Salem, A. Z. M., Elghandour, M. M. Y., Vázquez, J. F., Alonso, M. U., Barbabosa, A., Chagoyán, J. C. V. & Reyna, A. G. (2015). Oral administration of Sauce llorón extract to growing lambs to control gastrointestinal nematodes and Moniezia spp. Asian Pacific Journal of Tropical Medicine, 8(7), 520–525.
Chatsudthipong, V. & Muanprasat, C. (2009). Stevioside and related compounds: Therapeutic benefits beyond sweetness. Pharmacology & Therapeutics, 121(1), 41–54.
Cho, B. O., Ryu, H. W., So, Y., Cho, J. K., Woo, H. S., Jin, C. H., Seo, K. I., Park, J. C. & Jeong, I. Y. (2013). Anti-inflammatory effect of austroinulin and 6-O-acetyl-austroinulin from Stevia rebaudiana in lipopolysaccharide-stimulated RAW2647 macrophages. Food and Chemical Toxicology, 62, 638–644.
Cho, S., Mbiriri, D. T., Shim, K., Lee, A. L., Oh, S. J., Yang, J., Ryu, C., Kim, Y. H., Seo, K. S., Chae, J. I., Oh, Y. K. & Choi, N. J. (2014). The influence of feed energy density and a formulated additive on rumen and rectal temperature in Hanwoo steers. Asian-Australasian Journal of Animal Sciences, 27(11), 1652–1662.
Chu, Y., Chang, C. & Hsu, H. (2000). Flavonoid content of several vegetables and their antioxidant activity. Journal of the Science of Food and Agriculture, 80(5), 561–566.
Curi, R., Alvarez, M., Bazotte, R. B., Botion, L. M., Godoy, J. L. & Bracht, A. (1986). Effect of Stevia rebaudiana on glucose tolerance in normal adult humans. Brazilian Journal of Medical and Biological Research, 19(6), 771–774.
Daley, C. A., Abbott, A., Doyle, P. S., Nader, G. A. & Larson, S. (2010). A review of fatty acid profiles and antioxidant content in grass-fed and grain-fed beef. Nutrition Journal, 9, 1-12. https://doi.org/10.1186/1475-2891-9-10
Diasio, R. B. & LoBuglio, A. F. (1996). Immunomodulator: immunosuppressive agents and I immunostimulants. In: Goodman & Gilman’s, The Pharmacological Basis of Therapeutics, ed. by Hardman, J.G. and Limbird, L.E. The New York: McGraw-Hill, 9th Edition, 1291–1307.
Edeoga, H. O., Okwu, D. E. & Mbaebie, B. O. (2005). Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology, 4(7), 685–688.
EFSA FAF Panel (EFSA Panel on Food Additives and Flavourings) (2022). Safety evaluation of glucosylated steviol glycosides as a food additive in different food categories. EFSA Journal, Wiley-VCH GmbH, 20(2), 7066.
Enchev, S., Mehmed, A. & Kikidonov, G. (2018). Effect of mineral and organic fertilization on the production of Stevia (Stevia rebauduana B.). Bulg. J. Agric. Sci., 24 (Suppl. 2), 100–103.
Fahim, N. H., Kholif, A. E. & Azzaz, H. H. (2022). Fennel and ginger improved nutrient digestibility and milk yield and quality in early lactating Egyptian buffaloes. Annals of Animal Science, 22(1), 255–270.
FAO/WHO (Food and Agriculture Organization/World Health Organization) (2003). Diet, nutrition and prevention of chronic diseases. WHO Technical Report Series #916, Report of a joint WHO/FAO expert conclusion, Geneva 2003, 149.
Fengyang, L., Yunhe, F., Bo, L., Zhicheng, L., Depeng, L., Dejie, L., Wen, Z., Yongguo, C., Naisheng, Z., Xichen, Z. & Zhengtao, Y. (2012). Stevioside suppressed inflammatory cytokine secretion by downregulation of NF-κB and MAPK signaling pathways in LPS-stimulated RAW264.7 cells. Inflammation, 35, 1669–1675.
Fernandes, S. A. A., Mattos, W. R. S., Matarazzo, S. V., Tonhati, H., Gama, M. A. S. & Lanna, D. P. D. (2007). Total fatty acids in Murrah buffaloes milk on commercial farms in Brazil. Italian Journal of Animal Science, 6(Suppl. 2), 1063–1066.
Ferri, L. A., Alves-Do-Prado, W., Yamada, S. S., Gazola, S., Batista, M. R. & Bazotte, R. B. (2006). Investigation of the antihypertensive effect of oral crude stevioside in patients with mild essential hypertension. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives,20(9), 732–736.
Geuns, J. M., Augustijns, P., Mols, R., Buyse, J. G. & Driessen, B. (2003a). Metabolism of stevioside in pigs and intestinal absorption characteristics of stevioside, rebaudioside A and steviol. Food Chemistry and Toxicology, 41(11), 1599–1607.
Geuns, J. M., Malheiros, R. D., Moraes, V. M., Decuypere, E. M., Compernolle, F. & Buyse, J. G. (2003b). Metabolism of stevioside by chickens. Journal of Agricultural and Food Chemistry, 51(4),1095–1101.
Ghanta, S., Banerjee, A., Poddar, A. & Chattopadhyay, S. (2007). Oxidative DNA damage preventive activity and antioxidant potential of Stevia rebaudiani (Bertoni), a natural sweetener. Journal of Agricultural and Food Chemistry, 55(26), 10962–10967.
Ghosh, S., Subudhi, E. & Nayak, S. (2008). Antimicrobial assay of Stevia rebaudiana Bertoni leaf extracts against 10 pathogens. International Journal of Integrative Biology, 2(1), 27–31.
Givens, D. I. & Shingfield, K. J. (2006). Optimizing dairy milk fatty acid composition. In: Improving the Fat Content of Foods, ed. by Williams, C. and Buttriss, J., Woodhead Publishing Limited. Cambridge, 252–280.
Goyal, S. K., Samsher, G. R. & Goyal, R. K. (2010). Stevia (Stevia rebaudiana) a bio-sweetener: a review. International Journal of Food Sciences and Nutrition, 61(1), 1–10.
Gregersen, S., Jeppesen, P. B., Holst, J. J. & Hermansen, K. (2004). Antihyperglycemic effects of stevioside in type 2 diabetic subjects. Metabolism: Clinical and Experimental, 53(1), 73–76.
Guo, L. R., Su, Z., Kong, Z. W., Huang, J. F., Zhong, R. Y. & Guo, T. F. (2016). Effects of feeding fermented Stevia residue on amino acid and fatty acid composition in pig muscle. Pig Science, 33, 72-73.
Gupta, E., Purwar, S., Sundaram, S., Tripathi, P. & Rai, G. (2016). Stevioside and rebaudioside a - predominant ent-kaurene diterpene glycosides of therapeutic potential: a review. Czech Journal of Food Science, 34(4), 281–299.
Han, X., Chen, C., Zhang, X., Wei, Y., Tang, S., Wang, J., Tan, Z. & Xu, L. (2019). Effects of dietary stevioside supplementation on feed intake, digestion, ruminal fermentation, and blood metabolites of goats. Animals, 9(2), 32.
He, L., Ji, S. S., Zhang, Z. H., Wang, D. S., Guo, L. R., Zhang, G. H. & Ding, J. N. (2017). Effects of Andrographis paniculata residue and sweet leaf inulin residue on sheep rumen fermentation in vitro. Chinese Journal of Animal Husbandry, 53, 86–89.
Ilias, N., Hamzah, H., Ismail, I. S., Mohidin, T. B. M., Idris, F. & Ajat, M. (2021). An insight on the future therapeutic application potential of Stevia rebaudiana Bertoni for atherosclerosis and cardiovascular diseases. Biomedicine & Pharmacotherapy, 143. DOI: 10.1016/j.biopha.2021.112207.
Ilieva, Y., Mihaylova, D., Ilyazova, A. & Penchev, P., Abadjieva, D. & Kistanova, E. (2022). Effects of the herbal preparation AyuFertin, used for anestrus overcome, on fatty acids composition of milk in Bulgarian Murrah buffaloes. Bulgarian Journal of Veterinary Medicine, 25(3), 440-450.
Jiang, J., Qi, L., Lv, Z., Jin, S., Wei, X. & Shi, F. (2019). Dietary stevioside supplementation alleviates lipopolysaccharide-induced intestinal mucosal damage through anti-inflammatory and antioxidant effects in broiler chickens. Antioxidants, 8(12), 575.
Jiang, J. L., Qi, L. N., Dai, H. J., Hu, C. H, Lu, Z. P., Wei, Q. W. & Shi, F. X. (2020). Dietary stevioside supplementation improves laying performance and eggshell quality through increasing estrogen synthesis, calcium level and antioxidant capacity of reproductive organs in aged breeder hens. Animal Feed Science and Technology, 269, 114682.
Jiang, M., Datsomor, O., Cheng, Z., Meng, Z., Zhan, K., Yang, T., Huang, Y., Yan, Q. & Zhao, G. (2022). Partial substitution of alfalfa hay by Stevia (Stevia rebaudiana) hay can improve lactation performance, rumen fermentation, and nitrogen utilization of dairy cows. Frontiers in Veterinary Science, 9, 899148. DOI: 10.3389/fvets.2022.899148.
Kalač, P. & Samková, E. (2010). The effects of feeding various forages on fatty acid composition of bovine milk fat: a review. Czech Journal of Food Science, 55(12), 521–537.
Kasti, A. N., Nikolaki, M. D., Synodinou, K. D., Katsas, K. N., Petsis, K., Lambrinou, S., Pyrousis, I. A. & Triantafyllou, K. (2022). The effects of stevia consumption on gut bacteria: friend or foe? Microorganisms, 10(4), 744. DOI: 10.3390/microorganisms10040744.
Khattab, M. S. A., Kholif, A. E., Abd El Tawab, A. M., Shaaban, M. M., Hadhoud, F. I., El-Fouly, H. A. & Olafadehan, O. A. (2020). Effect of replacement of antibiotics with thyme and celery seed mixture on the feed intake and digestion, ruminal fermentation, blood chemistry, and milk lactation of lactating Barki ewes. Food & Function, 11(8), 6889–6898.
Kikindonov, Tz. (2013). Assessment of initial material for stevia (Stevia rebaudiana B.) breeding. Agricultural Science and Technology, 5(1), 22–24, 1313-8820.
Kikindonov, Tz. & Enchev, S. (2012). Assessment of seeds germination of stevia (Stevia rebaudiana B.) origins. Agricultural Science, 45(3), 18–23 (Bg).
Konoshima, T. & Takasaki, M. (2002). Cancer-chemopreventative effects of natural sweeteners and related compounds. Pure and Applied Chemistry, 74(7), 1309-1316.
Kurek, J. M., Mikołajczyk-Stecyna, J. & Krejpcio, Z. (2023). Steviol glycosides from Stevia rebaudiana Bertoni mitigate lipid metabolism abnormalities in diabetes by modulating selected gene expression – An in vivo study. Biomedicine & Pharmacotherapy, 166, 115424 DOI:10.1016/j.biopha.2023.115424.
Lemus-Mondaca, R., Vega-Galvez, A., Zura-Bravo, L. & Ah-Hen, K. (2012). Stevia rebaudiana Bertoni, source of a high-potency natural sweetener: a comprehensive review on the biochemical, nutritional and functional aspects. Food Chemistry, 132(3), 1121–1132.
Lemus-Mondaca, R., Vega-Galvez, A., Rojas, P., Stucken, K., Delporte, C., Valenzuela-Barra, G., Jagus, R. J., Agüer, M. V. & Pasten, A. (2018). Antioxidant, antimicrobial and anti-inflammatory potential of Stevia rebaudiana leaves: effect of different drying methods. Journal of Applied Research on Medicinal and Aromatic Plants, 11, 37–46.
Liu, S., Xiong, Y., Cao, S., Wen, X., Xiao, H., Li, Y., Chi, L., He, D., Jiang, Z. & Wang, L. (2022). Dietary stevia residue extract supplementation improves antioxidant capacity and intestinal microbial composition of weaned piglets. Antioxidants, 11(10), 2016. DOI: 10.3390/antiox11102016.
Ma, L. & Ma, Y. (2009). The comprehensive development and utilization of stevia. China Sugar, 1, 68–69.
Matloup, O. H., Abd El Tawab, A. M., Hassan, A. A., Hadhoud, F. I., Khattab, M. S. A., Khalel, M. S., Sallam, S. M. A. & Kholif, A. E. (2017). Performance of lactating Friesian cows fed a diet supplemented with coriander oil: Feed intake, nutrient digestibility, ruminal fermentation, blood chemistry, and milk production. Animal Feed Science & Technology, 226, 88–97.
Mehmed, A. & Enchev, S. (2020). Influence of organic foliar fertilizerson stevia development (Stevia rebaudiana B.). Journal of Mountain Agriculture on the Balkans, 23(4), 230-242.
Mehmed, A., Enchev, S. & Bozhimirov, S. (2021). Assessment of initial material for stevia selection (Stevia rebaudiana B.). Bulg. J. Agric. Sci., 27(3), 536–540.
Midmore, D. J. & Rank, A. H. (eds). (2002). A new rural industry – Stevia – to replace imported chemical sweeteners. A Report for the Rural Industries Research and Development Corporation. Barton, Queensland, Australia.
Mizushina, Y., Akihisa, T., Ukiya, M., Hamasaki, Y., Murakami-Nakai, C., Kuriyama, I., Takeuchi, T., Sugawara, F. & Yoshida, H. (2005). Structural analysis of isosteviol and related compounds as DNA polymerase and DNA topoisomerase inhibitors. Life Sciences, 77(17), 2127–2140.
Molina-Barrios, R. M., Avilés-Trejo, C. R., Puentes-Mercado, M. E., Cedillo-Cobián, J. R. & Hernández-Chavez, J. F. (2021). Effect of dietary stevia-based sweetener on body weight and humoral immune response of broiler chickens. Veterinary World, 14(4), 913–917.
Momtazi-Borojeni, A. A., Esmaeili, S., Abdollahi, E. & Sahebkar, A. (2017). A review on the pharmacology and toxicology of steviol glycosides extracted from Stevia rebaudiana. Current Pharmaceutical Design, 23(11), 1616–1622.
Muanda, F. N., Soulimani, R., Diop, B. & Dicko, A. (2011). Study on chemical composition and biological activities of essential oil and extracts from Stevia rebaudiana Bertoni leaves. LWT -Food Science and Technology, 44(9), 1865–1872.
Munro, P. J., Lirette, A., Anderson, D. M. & Ju, H. Y. (2000). Effects of a new sweetener, Stevia, on performance of newly weaned pips. Canadian Journal of Animal Science, 80(3), 529–531.
Nikolova-Damyanova, B., Bankova, V. & Popov, S. (1994). Separation and quantitation of stevioside and rebaudiside A in plant extracts by normal-phase high performance liquid chromatography and thin-layer chromatography: A comparison. Phytochemical Analysis, 5(2), 81–85.
Nogoy, K. M. C., Sun, B., Shin, S., Lee, Y., Zi Li, X., Choi, S. H. & Park, S. (2022). Fatty acid composition of grain- and grass-fed beef and their nutritional value and health implication. Food Science of Animal Resources, 42(1), 18-33.
Park, J. E. & Cha, Y. S. (2010). Stevia rebaudiana Bertoni extract supplementation improves lipid and carnitine profiles in C57BL/6J mice fed a highfat diet. J. Sci. Food Agric., 90(7), 1099–1105.
Parodi, P. W. (2004). Milk fat in human nutrition. Australian Journal of Dairy Technology, 59(1), 3.
Patwardhan, B., Kalbag, D., Patki, P. S., Nagsampagi, B. A. (1990). Search of immunomodulatory agents: a review. Indian Drugs, 28(2), 56-63.
Penchev, P., Ilieva, Y. & Ivanova, S. (2022). Effect of supplementation of curcumin to the diet of buffaloes on the fatty-acid profile of milk and the derivative yoghurt. Zhivotnovadni nauki, 59(4), 78–87 (Bg).
Pirgozliev, V., Whiting, I. M., Mansbridge, S. C., Enchev, S., Rose, S. P., Kljak, K., Johnson, A. E., Drijfhout, F., Orczewska-Dudek, S. & Atanasov, A. G. (2021a). Effect of rearing temperature on physiological measures and antioxidant status of broiler chickens fed stevia (Stevia rebaudiana B.) leaf meal and exogenous xylanase. Current Research in Biotechnology, 3, 173-181.
Pirgozliev, V., Kljak, K., Whiting, I. M., Rose, S. P., Mansbridge, S. C., Enchev, S., Atanasov, A. G. & Stringhini, H. (2021b). Feeding dry stevia leaf (Stevia rebaudiana) or xylanase improves the hepatic antioxidative status of broiler chickens. Research in Veterinary Science, 136, 227–229.
Pirgozliev, V., Whiting, I. M., Kljak, K., Mansbridge, S. C., Atanasov, A. G., Rose, S. P. & Enchev, S. (2022). Stevia (Stevia rebaudiana) improves carotenoid content in eggs when fed to laying hens. Foods, 11, 1418.
Puri, M., Sharma, D. & Tiwari, A. K. (2011). Downstream processing of stevioside and its potential applications. Biotechnology Advances, 29(6), 781–791.
Ramos-Tovar, E., Hernández-Aquino, E., Casas-Grajales, S., Buendia-Montaño, L. D., Galindo-Gómez, S., Camacho, J., Tsutsumi, V. & Muriel, P. (2018). Stevia prevents acute and chronic liver injury induced by carbon tetrachloride by blocking oxidative stress through Nrf2 upregulation. Oxidative Medicine and Cellular Longevity, 2018(1), 3823426. DOI: 10.1155/2018/3823426.
Rotimi, S. O., Rotimi, O. A., Adelani, I. B., Onuzulu, C., Obi, P. & Okungbaye, R. (2018). Stevioside modulates oxidative damage in the liver and kidney of high fat / low streptozocin diabetic rats. Heliyon, 4(5), e00640. DOI: 10.1016/j.heliyon. 2018.e00640
Salem, A. Z. M., Elghandour, M. M. Y., Kholif, A. E., López, S., Pliego, A. B., Cipriano-Salazar, M., Chagoyán, J. C. V., de Oca Jiménez, R. M. & Alonso, M. U. (2017). Tree leaves of Salix babylonica extract as a natural anthelmintic for small-ruminant farms in a semiarid region in Mexico. Agroforestry Systems, 91, 111–122.
Sarnataro, C. & Spanghero, M. (2020). In vitro rumen fermentation of feed substrates added with chestnut tannins or an extract from Stevia rebaudiana Bertoni. Animal Nutrition, 6(1), 54–60.
Savita, S. M., Sheela, K., Sunanda, S., Shankar, A. G., Ramakrishna, P. & Sakey, S. (2004). Health implications of Stevia rebaudiana. Journal of Human Ecology, 15(3), 191–194.
Scollan, N. D., Enser, M., Gulati, S.K., Richardson, I. & Wood, J. D. (2003). Effects of including a ruminally protected lipid supplement in the diet on the fatty acid composition of beef muscle. British Journal of Nutrition, 90(3), 709–716.
Sehar, I., Kaul, A., Bani, S., Pal, H. C. & Saxena, A. K. (2008). Immune up regulatory response of a non-caloric natural sweetener, stevioside. Chemico-Biological Interactions, 173(2), 115–121.
Shang, H. Q. (2011). Research progress on comprehensive utilization of Stevia. Biology Teaching, 36, 4–6.
Sharif, R., Chan, K. M., Ooi, T. C. & Mohammad, N. F. (2017). Cytotoxicity and genotoxicity evaluation of stevioside on CCD18Co and HCT 116 cell lines. International Food Research J., 24(1), 341–345.
Sharma, N., Mogra, R. & Upadhyay, B. (2009). Effect of stevia extract intervention on lipid profile. Studies on Ethno-Medicine, 3(2), 137–140.
Shin, Y. G., Rathnayake, D., Mun, H. S., Dilawar, M. A., Pov, S. & Yang, C. J. (2021). Sensory attributes, microbial activity, fatty acid composition and meat quality traits of Hanwoo cattle fed a diet supplemented with stevioside and organic selenium. Foods, 10(1), 129. DOI: 10.3390/foods10010129.
Shingfield, K. J., Salo-Väänänen, P., Pahkala, E., Toivonen, V., Jaakkola, S., Piironen, V. & Huhtanen, P. (2005). Effect of forage conservation method, concentrate level and propylene glycol on the fatty acid composition and vitamin content of cows´mik. Journal of Dairy Research, 72(3), 349–361.
Shivanna, N., Naika, M., Khanum, F. & Kaul, V. K. (2013). Antioxidant, anti-diabetic and renal protective properties of Stevia rebaudiana. Journal of Diabetes and its Complications, 27(2), 103–113.
Shukla, S. & Mehta, A. (2015). Comparative phytochemical analysis and in vivo immunomodulatory activity of various extracts of Stevia rebaudiana leaves in experimental animal model. Frontiers in Life Science, 8, 55-63.
Shukla, S., Mehta, A., Mehta, P. & Bajpai, V. K. (2012). Antioxidant ability and total phenolic content of aqueous leaf extract of Stevia rebaudiana Bertoni. Experimental and Toxicologic Pathology, 64(7-8), 807–811.
Simonyan, K. V., Chavushyan, V. A., Avetisyan, L. G., Simonyan, G. M., Hovhannisyan, L. E. & Simonyan, M. A. (2021). Regulatory effects of Stevia rebaudiana on NADPH oxidase-related manifestations of oxidative stress in diabetic rats with spinal cord injury. Neurophysiology, 53(1), 13–21.
Singh, S. & Rao, G. (2005). Stevia: The herbal sugar of 21st Century. Sugar Technology, 71(1),17–24.
Slavova, J., Nenkova, D. & Ivanova, I. (2003). Study on the benzymidazol upon Stevia rebaudiana Bertoni, cultivated in vitro. Bulg. J. Agric. Sci., 9(2), 225–228.
Smith, S. B., Clare, A. G., Lunt, D. K. & Brooks, M. A. (2009). Regulation of fat and fatty acid composition in beef cattle. Asian-Australian Journal of Animal Science, 22(9), 1225–1233.
Takahashi, K., Matsuda, M., Ohashi, K., Taniguchi, K., Nakagomi, O., Abe, Y., Mori, S., Sato, N., Okutani, K. & Shigeta, S. (2001). Analysis of anti-rotavirus activity of extract from Stevia rebaudiana. Antiviral Research, 49(1), 15–24.
Takasaki, M., Konoshima, T., Kozuka, M., Tokunda, H. & Takayasu, J. (2009). Cancer preventive agents. Part 8: Chemopreventive effects of stevioside and related compounds. Bioorganic & Medicinal Chemistry, 17(2), 600–605.
Tanova, K. & Kaschieva, M. (2018). Testing alternative control means for the pathogenic fungus Alternaria alternate f. ssp. stevae, isolated from stevia – Stevia rebaudiana Bertoni. Journal of Mountain Agriculture on the Balkans, 21(6), 214–223.
Thomas, J. & Glade, M. (2010). Stevia: It’s not just about calories. Open Obesity Journal, 2, 101–109.
Tomita, T., Sato, N., Arai, T., Shiraishi, H., Sato, M., Takeuchi, M. & Kamio, Y. (1997). Bactericidal activity of a fermented hot-water extract from Stevia rebaudiana Bertoni towards enterohemorrhagic Escherichia coli O157:H7 and other food-borne pathogenic bacteria. Microbiology and Immunology, 41(12), 1005–1009.
Uchkunov, V., Uchkunova, K. & Uchkunov, I. (2015). Effect of the selection on the degree of resistance to Alternaria alternata f. ssp. steviae in breeding origins of stevia (Stevia rebaudiana B.). Plant Science, 52(1), 36–38 (Bg).
Wang, Y. X., Zhou, G. L., Liao, Z. Y., Xu, W., Dong, Q. G. & Wang, T. (2011). Study on the application of stevioside in weaning piglets. China Feed, 4, 29-31.
Wang, L. S., Shi, Z., Shi, B. M. & Shan, A. S. (2014). Effects of dietary stevioside/rebaudioside A on the growth performance and diarrhea incidence of weaned piglets. Animal Feed Science & Technology, 187, 104–109.
Wolwer-Rieck, U. (2012). The leaves of Stevia rebaudiana (Bertoni), their constituents and the analyses thereof: a review. Journal of Agricultural and Food Chemistry, 60(4), 886–895.
Xi, Y., Yamaguchi, T., Sato, M. & Takeuchi, M. (1998a). Antioxidant activity of Stevia rebaudiana. The Japanese Society for Food Science and Technology, 45(5), 310-316.
Xi, Y., Yamaguchi, T., Sato, M. & Takeuchi, M. (1998b). Antioxidant mechanism of Stevia rebaudiana extract and antioxidant activity of inorganic salts. The Japanese Society for Food Science and Technology, 45(5), 317–322.
Xie, F. Y. & Wang, G. J. (2010). Effects of adding stevia stalk in dairy cow diets on lactation performance of dairy cows. Fujian Journal of Animal Husbandry and Veterinary Medicine, 12, 106.
Yasukawa, K., Kitanaka, S. & Seo, S. (2002). Inhibitory effect of stevioside on tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in two stage carcinogenesis in mouse skin. Biological and Pharmaceutical Bulletin, 25(11), 1488-1490.
Yu, M., Tie, G., Liu, Z. & Diao, X. P. (2020). Effects of dietary supplementation with high fiber (Stevia residue) on the fecal flora of pregnant sows. Animals, 10(12), 2247 DOI: 10.3390/ani10122247.
Zhang, X., Jiao, T., Ma, S., Chen, X., Wang, Z., Zhao, S. & Ren, Y. (2023). Effects of different proportions of stevia stalk on nutrient utilization and rumen fermentation in ruminal fluid derived from sheep. Peer Journal, 11. e14689 DOI: 10.7717/peerj.14689.
Zhao, L., Yang, H., Xu, M., Wang, X., Wang, C., Lian, Y., Mehmood, A. & Dai, H. (2019). Stevia residue extract ameliorates oxidative stress in d-galactose-induced aging mice via Akt/Nrf2/HO-1 pathway. Journal of Functional Foods, 52, 587–595. |
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| Date published: 2025-06-24
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