EFFECTS OF ORGANIC ADDITIVES AND NAPHTHALENE ACETID ACID (NAA) APPLICATION ON THE IN VITRO GROWTH OF BLACK ORCHID HYBRID (COELOGYNE PANDURATA LINDLEY)

Ongko Cahyono, Retna Bandriyati Arniputri, Lili Anatus Soliah, Sri Hartati

Abstract: Black orchid (Coelogyne pandurata), Bornean native beautiful orchid, which is currently threatened with extinction, was successfully crossed with Coelogyne rumphii. The hybrids will be multiplicated using in vitro propagation technique. To produce plants with good results both quantitatively and qualitatively it is necessary to add the growth regulator substances and organic additive compound into the culture medium. This study aim to get the best medium composition that is able to optimize the growth protocol for the hybrids of Coelogyne pandurata and Coelogyne rumphii. The experiment was arranged in a factorial design based on completely randomized design with two factors and fi ve replications. The first factor was the concentration of Naphthalene Acetic Acid /NAA (0, 1, 3 and 5 ppm). The second factor was the organic additivess (without any organic additives, coconut water was 250 ml L-1, banana 150 g L-1, potato 200 g L-1 and sweet potato 150 g L-1). The results showed that the addition of 3 ppm NAA capable of accelerating time root emergence. The organic materials affect all of parameter significantly. The combination of 3 ppm NAA with organic coconut water can accelerate the emergence of shoots of 9.8 days after planting (DAP), combination of 1 ppm NAA with coconut water can stimulate the multiplication of shoots as many as 6.29 shoots and combination of 3 ppm NAA with sweet potatoes can increase the number of roots as many as 23.5 roots.

Keywords: Coelogyne pandurata; Coelogyne rumphii; composition medium; genetic diversity; in vitro

Date published: 2018-01-15