Specialized trait collections for the different selection directions in cotton.  II. Specialized trait collections based on exceptional characteristics for ecological cotton production and heterosis selection

Neli Valkova; Valentina Dimitrova; Minka Koleva; Spasimira Nedyalkova

Specialized trait collections for the different selection directions in cotton. II. Specialized trait collections based on exceptional characteristics for ecological cotton production and heterosis selection

Neli Valkova

, Valentina Dimitrova

, Minka Koleva

, Spasimira Nedyalkova

Abstract: Specialized trait collections based on exceptional characteristics for ecological cotton production and heterosis selection have been created: specialized collection based on naturally colored cotton (brown and green); specialized collection based on naked seed genotypes of cotton; specialized collection based on forms with male sterility. Organization of the relevant collections was in accordance with the requirements of selection process for a complex assessment of biological and economic qualities, adaptability to different environmental conditions, resistance to diseases and enemies. Created collections will facilitate the use of available genetic resources in cotton breeding to solve some existing ecological problems in the production and heterosis selection, to create varieties with high economic and ecological effects, and high-yielding hybrids based on male sterility. On the other hand, with them, commercial and other available germplasm will be preserved and the hereditary basis of cotton selection in the respective directions will be enriched and expanded.

Keywords: colored cotton; cytoplasmic male sterility; genetic resources; naked seed

Citation: Valkova, N., Dimitrova, V., Koleva, M. & Nedyalkova, S. (2026). Specialized trait collections for the different selection directions in cotton. II. Specialized trait collections based on exceptional characteristics for ecological cotton production and heterosis selection. Bulg. J. Agric. Sci., 32(6), 601–609

References: (click to open/close)

Ahmad, A., Farooq, J., Chattha, W. S. & Naveed-Ul-Haq, M. (2014). Association of qualitative and yield contributing traits in upland cotton. Agriculture & Forestry, 60(1), 115 - 123.
Du XiongMing, D. X., Liu GuoQiang, L. G., Shi YuZhen, S. Y., Zhou ZhongLi, Z. Z., Fu HaiQin, F. H., Qiang AiDi, Q. A. & Li RunHua, L. R. (2000). Coloured cotton variety - Brown Fiber 1 and the key technique for its selection. China Cottons, 27(7), 10 - 12.
Garcia, L., Edera, A., Marfil, C. & Sanchez-Puerta, V. (2019). Male sterility and somatic hybridization in plant breeding. Rev. FCA UNCUYO, 51(2), 475 - 486.
Geng, J. Y., Wang, G. Y., Zhai, X. J., Li, Z. S., Liu, C. J., Li, Y. Z., Geng, J. Y., Wang, G. Y., Zhai, X. J., Li, Z. S., Liu, C. J. & Li, Y. Z. (1998). Effect of coloured fiber gene on economic properties of upland cotton and analysis of its inheritance. Acta Gossypir Sinica, 10(6), 307 - 311.
Gülümser, T. (2016). Comparison between naturally colored cotton fabric and white cotton fabric in manner of processes in the dyehouse. Tekstil ve Konfeksiyon, 26(3), 287 - 294.
Han, X., Gao, C., Liu, L., Zhang, Y., Jin, Y., Yan, Q., Yang, L., Li, F. & Yang, Z. (2022). Integration of eQTL Analysis and GWAS Highlights Regulation Networks in Cotton under Stress Condition. International journal of molecular sciences, 23(14), 7564. https://doi.org/10.3390/ijms23147564.
Hidayat, T. S., Nurindah & Sunarto, D. A. (2020). Developing of Indonesian colored cotton varieties to support sustainable traditional woven fabric industry. IOP Conf. Ser.: Earth Environ. Sci., 418, 012073. DOI 10.1088/1755-1315/418/1/012073
IASAS (Executive Agency for Variety Testing, Approbation and Seed Control) - Main Directorate "Variety Testing, Approbation and Seed Control", Department "Variety Testing for Distinctiveness, Homogeneity and Stability and Biological and Economic Qualities" (2013, 2015 and 2016). Results of the Variety Test for Biological, Economic and Technological Qualities of Cotton (white cotton) (Bg).
Kaul, M. (1998). Male Sterility in Higher Plants. Springer Berlin, Heidelberg, 1005.
Keshamma, E., Manjula, A. C., Prathibha, K. Y. & Lolita, J. C. (2021). Colour Cotton Research: Current and Future Perspectives. International Journal of Creative Research Thoughts, 9(9), 384 - 388.
Khan, A. H., Min, L., Ma, Y., Wu, Y., Ding, Y., Li, Y., Xie, S., Ullah, A., Shaban, M., Manghwar, H., Shahid, M., Zhao, Y., Wang, C. & Zhang, X. (2020). High day and night temperatures distinctively disrupt fatty acid and jasmonic acid metabolism, inducing male sterility in cotton. J. Exp. Bot., 71(19), 6128 - 6141. doi: 10.1093/jxb/ eraa319.
Kong, X., Liu, D., Liao, X., Zheng, J., Diao, Y., Liu, Y. & Zhoi, R. (2017). Comparative analysis of the cytology and transcriptomes of the cytoplasmic male sterility line H276A and its maintainer line H276B of cotton (Gossypium barbadense l.). Int. J. Mol. Sci., 18(11), 2240. doi: 10.3390/ijms18112240.
Leonard, P. S., Dugger, P. & Richer, D. (1999). New crop in Pensilvania: Gossypium hirsutum L. research for improved fiber strength, shortened growing season, and increase wax content. Proceedings Beltwide Cotton Conferences, Orlando, Florida, USA, 3-7 Jan., 1, 499 - 500.
Lecture 7. (2021). Male sterility & its significance in plant breeding. Mustansiriyah Univer-sity, Baghdad,Iraq. https://www.uomustansiriyah.edu.iq/media/lectures/6/6_2021_09_15!06_37_26_PM.docx.
Li, S., Chen, Z., Zhao, N., Wang, Y., Nie, H. & Hua, J. (2018). The comparison of four mitochondrial genomes reveals cytoplasmic male sterility candidate genes in cotton. BMC Genomics, 19(1), 1 - 15. doi: 10.1186/s12864-018-5122-y.
Li, Y., Qin, T., Wei, C., Sun, J., Dong, T., Zhou, R., Chen, Q. & Wang, Q. (2019). Using transcriptome analysis to screen for key genes and pathways related to cytoplasmic male sterility in cotton (Gossypium hirsutum L.). Int. J. Mol. Sci., 20(20), 5120. doi: 10.3390/ijms20205120.
Li, M., Chen, L., Khan, A., Kong, X., Khan, M. R., Rao, M. J., Wang, J., Wang, L. & Zhou, R. (2021). Transcriptome and MiRNAomics analyses identify genes associated with cytoplasmic male sterility in cotton (Gossypium hirsutum L.). Int. J. Mol. Sci., 22(9), 4684. doi: 10.3390/ijms22094684.
Li, Y., Li, Y., Su, Q., Wu, Y., Zhang, R., Li, Y., Ma, Y., Ma, H., Guo, X., Zhu, L., Min, L. & Zhang, X. (2022). High temperature induces male sterility via MYB66–MYB4–Casein kinase I signaling in cotton. Plant Physiol, 189(4), 2091 - 2109. doi: 10.1093/plphys/kiac213.
Ma, Y., Min, L., Wang, J., Li, Y., Wu, Y., Hu, Q., Ding, Y., Wang, M., Liang, Y., Gong, Z., Xie, S., Su, X., Wang, C., Zhao, Y., Fang, Q., Li, Y., Chi, H., Chen, M., Khan, A.H., Lindsey, K., Zhu, L., Li, X. & Zhang, X. (2021). A combination of genome-wide and transcriptome-wide association studies reveals genetic elements leading to male sterility during high temperature stress in cotton. New Phytol., 231(1), 165 - 181. doi: 10.1111/nph.17325.
Meyer, V. G. (1969). Some effects of genes, cytoplasm and environment on male sterility of cotton (Gossypium). Crop Science, 9, 237 - 242.
Meyer, V. G. (1973). Fertility-restorer genes for cytoplasmic male sterility from Gossypium harknessii - Beltwide Cotton Prod. Res. Conf. Proc., 65.
Meyer, V. G. (1975). Male sterility from Gossypium harknessii. J. Heredity, 66, 23 - 27.
Morales, K. Y., Bridgeland, A. H., Hake, K. D., Udall, J. A., Thomson, M. J. & Yu, J. Z. (2022). Homology-based identification of candidate genes for male sterility editing in upland cotton (Gossypium hirsutum L.). Front. Plant Sci., 13, 1006264. doi: 10.3389/fpls.2022.1006264.
Muqaddim, N. (2022). Study on the comparison of naturally coloured cotton and traditional off white fibre in manner of process in the apparel industry. International Journal of Advances in Engineering and Management, 4(11), 242 - 249.
Nie, H., Wang, Y., Su, Y. & Hua, J. (2018). Exploration of miRNAs and target genes of cytoplasmic male sterility line in cotton during flower bud development. Funct. Integr. Genomics, 18(4), 457 - 476. doi: 10.1007/s10142-018-0606-z.
Nirania, K. S., Chhabra, B. S. & Dutt, Y. (2004). Heterosis for yield and quality traits in genetic male sterility based upland cotton hybrids. Journal of Cotton Research and Development, 18(2), 132 - 136.
Raja, D., Kumar, S. M., Devi, R. P., Loganathan, S., Ramya, K., Kannan, N. & Subramanian, V. (2018). Identification of molecular markers associated with genic male sterility in tetraploid cotton (Gossypium hirsutum L.) through bulk segregant analysis using a cotton SNP 63K array. Czech J. Genet. Plant Breed., 54(4), 154 - 160.
Singh, S. B. (2006). Genetic variation for floral, architectural and economic traits in cytoplasmic male sterile lines of upland cotton. Journal of Soils and Crops, 16(2), 316 - 323.
Singh, P., Singh, V. V. & Waghmare, V. N. Naturally coloured cotton. Central Institute for Cotton Research Nagpur, CICR Technical Bulletin, 4, 1 – 10. https://cicr.org.in/wp-content/uploads/naturally_colored_cotton.pdf.
Singh, S. B., Singh, P. & Mayee, C. D. Male sterility in cotton. Central Institute for Cotton Research Nagpur, CICR Technical Bulletin, 24, 1 - 16, https://cicr.org.in/wp-content/uploads/TC_BL_26.pdf.
Solanke, A., Deshmukh, S. B., Mhasal, G. S. & Marawar, M. W. (2015). Heterosis and combining ability in genetic male sterility based diploid cotton hybrids for yield, yield component, fibre quality characters and oil content. Electronic Journal of Plant Breeding, 6(1), 150 - 156.
Stewart, J. M. (1999). A new cytoplasmic male sterile and restorer for cotton - In: Proc. of Beltwide Cotton Prod. Res. Conf., Natl. Cotton Council of Am., Memphis TN., 160 - 163.
Stoilova, A. (2008). Inheritance of fiber length and lint percentage in male sterility based F1 cotton hybrids. Rastenievadni nauki, 45, 504 - 509. (In Bulgarian with English abstract).
Stoilova, A. (2009). Inheritance of productivity in F1 cotton hybrids based on cytoplasmic male sterility. Field Crops Studies, 5(2), 315 - 320 (In Bulgarian with English abstract).
Stoilova, A. & Rusev, V. (2007). Biological and agronomic characters of male sterility cotton lines. In: International Scientific Conference "Plant Genetic Stoks – The Basis of Agriculture Today", June 13-14, 2007, Sadovo Topic Genetics, Breeding, Plant Biotechnology, 93 - 95.
Stoilova, A., Rusev, V. & Spasova, D. (2006). Male sterility in cotton and possibilities for its utilization. In: Year book, vol. VI, Goce Delcev University – Stip, Faculty of Agriculture, 7 - 16. UDS 63 (058), ISSN 1409-987X.
Stoilova, A., Saldzhiev, I. & Spasova, D. (2008). Combinative ability for productivity of some cotton male-sterile lines and varieties. In: Scientific conference with international participation "Science in the conditions of globalization". Scientific Papers, Volume III, Part II, 77 - 81. Ed. "Rainbow-YK" Kardzhali.
Stoilova, A., Terziev, Zh. & Saldzhiev, I. (2009). Breeding of brown cotton in Bulgaria. In: 10th International Cotton Conference: Natural Fibers - Their Attractiveness in Multi-Directional Applications. Gdynia, September 3-4, 2009. Monograph, Chapter II: Natural fibers – their cultivation, properties and applications, 87 - 91.
Stoilova, A., Terziev, J. & Saldzhiev, I. (2010). Izabell – a new brown cotton variety. Rastenievadni nauki, 47(1), 92 - 96 (Bg).
Stoilova, A., Saldzhiev, I. & Terziev, Zh. (2011). Productive and quality characteristics of brown cotton. Agricultural Science and Technology, 3(2), 88 - 93.
Tuteja, O. P., Kumar, S. & Luthra, P. (2004). Variability, heritability and genetic advance studies in CMS based hybrids in upland cotton (Gossypium hirsutum L.). Journal of Cotton Research and Development, 18(1), 42 - 43.
Tuteja, O. P., Kumar, S., Verma, S. K. & Singh, M. (2005). Heterosis for yield and its component traits in American cotton hybrids (Gossypium hirsutum L.) based on GMS system. National Journal of Plant Improvement, 7(2), 110 - 114.
Xuede, W., Yunguo, Z., Dutt, Y. & Xiyuan, N. (2004). Development of cytoplasmic male sterility-based hybrids of upland cotton (Gossypium hirsutum) in China. Indian Journal of Agricultural Sciences, 74(10), 529 - 533.
Wu, J., Gong, Y., Cui, M., Qi, T., Guo, L., Zhang, J. & Xing, Ch. (2011). Molecular characterization of cytoplasmic male sterility conditioned by Gossypium harknessii cytoplasm (CMS-D2) in upland cotton. Euphytica, 181(1), 17 - 29. doi: 10.1007/s10681-011-0357-6.
You, J., Li, M., Li, H., Bai, Y., Zhu, X., Kong, X., Chen, X. & Zhou, R. (2022). Integrated methylome and transcriptome analysis widen the knowledge of cytoplasmic Male sterility in cotton (Gossypium barbadense L.). Front. Plant Sci,. 13. doi: 10.3389/fpls.2022.770098.
Zhang, M., Zhang, X., Guo, L., Qi, T., Liu, G., Feng, J., Shahzad, K., Zhang, B., Li, X., Wang, H., Tang, H., Qiao, X., Wu, J. & Xing, C. (2020). Single-base resolution methylome of cotton cytoplasmic male sterility system reveals epigenomic changes in response to high-temperature stress during anther development. J. Exp. Bot., 71(3), 951 - 969. doi: 10.1093/jxb/erz470.
Zhang, M., Wei, H., Liu, J., Bian, Y., Ma, Q., Mao, G., Wang, H., Wu, A., Zhang, J., Chen, P., Ma, L., Fu, X. & Yu, S. (2021). Nonfunctional GoFLA19s are responsible for the male sterility caused by hybrid breakdown in cotton (Gossypium spp.). Plant J., 107(4), 1198 - 1212. doi: 10.1111/tpj.15378.
Zhang, J., Wu, P., Li, N., Xu, X., Wang, S., Chang, S., Zhang, Y., Wang, X., Liu, W., Ma, Y., Manghwar, H., Zhang, X., Min, L. & Guo, X. (2023). A male-sterile mutant with necrosis-like dark spots on anthers was generated in cotton. Front. Plant Sci., 13, 1102196. doi: 10.3389/fpls.2022.1102196.

Date published: 2026-06-25

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