IN VITRO CULTIVATION AND EX VITRO ADAPTATION OF NEPETA NUDA SSP. NUDA CORRELATION BETWEEN REGENERATION POTENTIAL, LEAF ANATOMY, AND PLASTID PIGMENTS

D. Dragolova, V. Kapchina-Toteva, D. Koleva, M. Stefanova, M. Zhiponova, M. Dimitrova

Abstract: The leaf anatomy and the amount of the plastid pigments in in vivo plants, in vitro plants, in vitro regenerated after cryopreservation plants (cryo plants) and ex vitro adapted plants of Nepeta nuda ssp. nuda were examined as markers for the assessment of the regeneration potential of the species. The anatomical study showed that under in vitro conditions leaf with thin lamina and uniformly structured assimilation parenchyma was formed. The leaf of in vivo and ex vitro plants was equally structured - bifacial. It was found that the amount of the plastid pigments is greater in in vitro, cryo and ex vitro plants as compared to in vivo plants. The highest value was measured in cryo plants. The results indicated a high regeneration potential of N. nuda ssp. nuda during in vitro cultivation and ex vitro adaptation.

Keywords: assimilation parenchyma; carotenoids; chlorophyll; cryopreservation; medicinal plant

Date published: 2017-09-20