Biotechnological approaches for the conservation of old local plum and grapevine genotypes
Lilyana Nacheva

, Nataliya Dimitrova

, Svetoslav Malchev

, Marko Mihaylov

Abstract: Conservation of plant biodiversity is of great importance in the face of changing climate conditions. The Balkan Peninsula is rich in valuable genetic resources of fruit species. At the Fruit Growing Institute – Plovdiv (Bulgaria), different biotechnological approaches have been applied to preserve and enrich the biodiversity. A new project to identify and protect local varieties and forms of fruit species and grapevines began three years ago. Two plum (Prunus spp.) and three grapevine (Vitis spp.) genotypes have been successfully introduced into in vitro culture. Reliable micropropagation protocols have been developed for these valuable genotypes, and they have been included in the in vitro gene bank. Plum and grapevine plants were successfully acclimatized to ex vitro conditions and stored in the ex situ collection at the Fruit Growing Institute - Plovdiv. This would facilitate their study, transportation, and further practical applications for germplasm conservation and genetic improvement of this species, as well as their inclusion in future breeding programs.
Keywords: biodiversity; micropropagation; plant genetic resources; rootstock; tissue culture
Citation: Nacheva, L., Dimitrova, N., Malchev, S. & Mihaylov, M. (2025). Biotechnological approaches for the conservation of old local plum and grapevine genotypes. Bulg. J. Agric. Sci., 31(5), 956–963
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| Date published: 2025-10-28
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