Influence of Immunobeta and Silymarin preparations on weight development and some indicators of oxidative stress in Lacaune lambs
Nadya Bozakova
, Lilyan Sotirov
, Miroslav Simeonov
, Veselin Ivanov
, Tsvetoslav Koynarski
, Krum Nedelkov
Abstract: Since 2006, the European Community has banned the use of food antibiotics as growth promoters in animal husbandry. The use of modern systems to produce animal products with a high degree of concentration and automation, has led to a deterioration of the health status of sensitive breeds and hybrids. The weight development of lambs of the Lacaune breed was studied for seven months. The immunomodulator Immunobeta® and the hepatoprotector Silymarin were found to improve growth reliably, increase iron-reducing plasma capacity (FRAP), and reliably lower malondialdehyde MDA and oxidative stress index (OSI) concentrations in lambs, both through thermoneutral and during a hot summer period.
Keywords: Immunobeta; lambs; oxidative stress; Silymarin
Citation: Bozakova, N., Sotirov, L., Simeonov, M., Ivanov, V., Koynarski, Ts. & Nedelkov, K. (2025). Influence of Immunobeta ® and Silymarin preparations on weight development and some indicators of oxidative stress in Lacaune lambs. Bulg. J. Agric. Sci., 31(4), 807–813.
| References: (click to open/close) | Alhidary, I. A., Rehman, Z., Khan, R. U. & Tahir, M. (2017). Anti-aflatoxin activities of milk thistle (Silybum marianum) in broiler. World’s Poultry Science Journal, 73, 559 - 566.
Andreeva, L. I., Kozhemyakin, L. A. & Kishkun, A. A. (1988). A modified thiobarbituric acid test for measuring lipid peroxidation. Lab Delo, 11, 41 - 43.
Armstrong, D. & Browne, R. (1994). The analysis of free radicals, lipid peroxides, antioxidant enzymes and com¬pounds related to oxidative stress as applied to the clinical chemistry laboratory. Advances in Experimental Medicine and Biology, 366, 43 - 58.
Atanassof, Al. (2016). Study of the influence of the preparation Vitasil on the diet of carp (Cyprinus carpio) grown in a recirculation system. Dissertation for the award of educational and scientific degree Doctor, Trakia University, Stara Zagora, (Bg).
Benzie, I. F. F. & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry, 239, 70 - 76.
Chaucheyras, F., Fonty, G., Bertin, G. & Gouet, P. (1995). Effects of live Saccharomyces cerevisiae cells on zoospore germination, growth, and cellulolytic activity of the rumen anaerobic fungus, Neocallimastix frontalis MCH3. Current Microbiology, 31(4), 201 - 205.
Chaucheyras, F., Fonty, G., Bertin, G. & Gouet, P. (1995). In vitro H2 utilization by a ruminal acetogenic bacterium cultivated alone or in association with an archaea methanogen is stimulated by a probiotic strain of Saccharomyces cerevisiae. Applied and Environmental Microbiology, 61(9), 3466 - 3467.
Chaucheyras-Durand, F. & Fonty, G. (2008). Establishment of cellulolytic bacteria and development of fermentative activities in the rumen of gnotobiotically-reared lambs receiving the microbial additive Saccharomyces cerevisiae CNCM I-1077. Reproduction Nutrition Development, 41(1), 57 - 68.
Chaucheyras-Durand, F., Ameilbonne, A., Auffret, P., Bernard, M., Mialon, M. M., Dunière, L. & Forano, E. (2019). Supplementation of live yeast based feed additive in early life promotes rumen microbial colonization and fibrolytic potential in lambs. Scientific Reports, 9(1), 19216.
Fiems, L. O., Cottyn, B. G., Dussert, L. & Vanacker, J. M. (1993). Effect of a viable yeast culture on digestibility and rumen fermentation in sheep fed different types of diets. Reproduction Nutrition Development, 33(1), 43 - 49.
Fanoudi, S., Mohaddeseh, S. A., Gholamreza, K. & Hossein, H. (2020). Milk thistle (Silybum Marianum) as an antidote or a protective agent against natural or chemical toxicities: a review. Drug and Chemical Toxicology, 43(3), 240 - 254.
Grela, E. R., Świątkiewicz, M., Florek, M. & Wojtaszewska, I. (2020). Impact of milk thistle (Silybum marianum L.) seeds in fattener diets on pig performance and carcass traits and fatty acid profile and cholesterol of meat, backfat and liver. Livestock Science, 239, 104 - 180.
Hooper, K. J., Bobe, G., Vorachek, W. R., Bishop-Stewart, J. K., Mosher, W. D., Pirelli, G. J., Kent, M. L. & Hall, J. A. (2014). Effect of selenium yeast supplementation on naturally acquired parasitic infection in ewes. Biological Trace Element Research, 161(3), 308 - 317.
Jia, P., Cui, K., Ma, T., Wan, F., Wang, W., Yang, D., Wang, Y., Guo, B., Zhao, L. & Diao, Q. (2018). Influence of dietary supplementation with Bacillus licheniformis and Saccharomyces cerevisiae as alternatives to monensin on growth performance, antioxidant, immunity, ruminal fermentation and microbial diversity of fattening lambs. Scientific Reports, 8(1), 16712. doi: 10.1038/s41598-018-35081-4.
Karvellas, C. J., Tillman, H., Leung, A. A., Lee, W. M., Schilsky, M. L., Hameed, B., Stravitz, R. T., McGuire, B. M. & Fix, O. K. (2016). Acute liver injury and acute liver failure from mushroom poisoning in North America. Liver International, 36(7), 1043 - 1050.
Khamisabadi, H. (2020). Effects of Silymarin on milk production, liver enzymes, oxidative status and HSP70 gene expression in postparturient Sanjabi ewes. Cellular and Molecular Biology, 66, 76 - 81.
Kim, D. H., Kim, K. H., Nam, I. S., Lee, S. S., Choi, C. W., Kim, W. Y., Kwon, E. G., Lee, K. Y., Lee, M. J. & Oh, Y. K. (2013). Effect of indigenous herbs on growth, blood metabolites and carcass characteristics in the late fattening period of hanwoo steers. Asian-Australas Journal of Animal Scienses, 26(11),1562 - 1568.
Kosina, P., Dokoupilová, A., Janda, K., Sládková, K., Silberová, P., Pivodová, V. & Ulrichová, J. (2017). Effect of Silybum marianum fruit constituents on the health status of rabbits in repeated 42-day fattening experiment. Animal Feed Science and Technology, 223, 128 - 140.
Oelrichs, P. (1982). Sawfly poisoning of cattle. Queensland Agricultural Journal, 108, 110 - 112.
Pickova, D., Ostry, V., Toman, J. & Malir, Fr. (2020). Presence of Mycotoxins in Milk Thistle (Silybum marianum) Food Supplements: A Review. Toxins, 8, 782.
Reis, L. F., Sousa, R. S., Oliveira, F. L. C., Rodrigues, F. A. M. L., Araújo, C. A. S. C., Meira-Júnior, E. B. S., Barrêto-Júnior, R. A., Mori, C. S., Minervino, A. H. H. & Ortolani, E. L. (2018). Comparative assessment of probiotics and monensin in the prophylaxis of acute ruminal lactic acidosis in sheep. BMC Veterinary Research, 14(1), 9.
Sahin, A., Arici, M. A., Yilmaz, Y., Kalkan, S., Durmus, N., Ergur, B. U., Yakut, A. I., Atabey, N. & Tuncok, Y. A. (2018). Comparison of the Effectiveness of Silibinin and Resveratrol in Preventing Alpha-Amanitin-Induced Hepatotoxicity. Basic & Clinical Pharmacology & Toxicology, 122, 633 - 642.
Silberberg, M., Chaucheyras-Durand, F., Commun, L., Mialon, M. M., Monteils, V., Mosoni, P., Morgavi, D. P. & Martin, C. (2013). Repeated acidosis challenges and live yeast supplementation shape rumen microbiota and fermentations and modulate inflammatory status in sheep. Animal, 7(12), 1910 - 1920.
Stone, C. A., Kawai. K., Kupka. R. & Fawzi. W. W. (2010). Role of selenium in HIV infection. Nutrition Reviews, 68, 671 - 681.
Thamsborg, S., Jorgensen, J., Brummerstedt, E. & Bjerregard, J. (1996). Putative effect of silymarin on sawfly (Arge pullata) induced hepatotoxicosis in sheep. Veterinary and Human Toxicology, 38, 89 91.
Uchiyama, M. & Michara, M. (1978). Determination of malondialdehyde precursor in tissues by thiobarbituric acid test. Biochemistry, 86, 271 - 278.
Urbanczyk, J., Hanczakowska, E. & Swiatkiewicz, M. (2002). Herb mixture as an antibiotic substitute in pig feeding. Medycyna Weterynaryjna, 58, 887 – 889 (Pl). |
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| Date published: 2025-08-27
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