Characterization of siderophore-producing bacteria isolated from plant rhizosphere
Pham Hong Hien
, Bui Huong Quynh, Vu Thi Linh, Le Quang Man, Duong Thi Nguyen
, Nguyen Van Giang
Abstract: Siderophores are synthesized by plants and microorganisms that thrive in low-iron conditions, serving as biocontrol agents and other applications in agriculture. The aim of the present study was to isolate, characterize, and optimize siderophore-producing bacterial strains DT9, DT10, and DT12. The 16S rDNA nucleotide sequence analysis revealed that DT9 and DT10 were closely related to Klebsiella pneumoniae, and DT12 was related to Acinetobacter bereziniae. All three strains produced siderophores, crucial compounds aiding plants in acquiring iron when growing in iron-scarce environments. The presented results in this study constituted preliminary findings evaluating the influences of cultural conditions on siderophore synthesis by DT9, DT10, and DT12. They demonstrated efficient siderophores synthesis when cultured at 30°C, with a culture medium pH of 7. Glycerol and (NH4)2SO4 served as the carbon and nitrogen sources for the culture medium.
Keywords: Acinetobacter bereziniae; carbon sources; Klebsiella pneumoniae; nitrogen sources; rhizospheric bacteria; siderophores
Citation: Hien, P. H., Quynh, B. H., Linh, V. T., Man, L. Q., Nguyen, D. T. & Giang, N. V. (2025). Characterization of siderophore-producing bacteria isolated from plant rhizosphere. Bulg. J. Agric. Sci., 31(2), 372–378.
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| Date published: 2025-04-28
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