Effect of genotypic variation in the FABP3 gene on milk productivity and fertility in Awassi ewes
Ivona Dimitrova
, Nevyana Stancheva
, Milena Bozhilova-Sakova
, Todor Tzonev
Abstract: A small family of low-molecular-weight intracellular fatty acid-binding proteins (FABPs), influence the transport and metabolism of long-chain fatty acids in the cell, as among them is fatty acid-binding protein 3 (FABP3). The aim of the study was to investigate the effect of genotypic variation in SNP3 of the FABP3 gene on milk productivity and fertility in Awassi sheep. The study included 61 ewes of different ages from a private herd in the village of Sredkovets, Shumen district, Bulgaria. Animal genotypes were established using PCR-RFLP analysis with application of restriction enzyme BseDI and subsequent gel electrophoresis. The presence of two alleles was found - the wild allele A (with fragments - 36 bp and 186 bp) and the mutant allele G (with fragments of 36 bp, 43 bp and 143 bp) with frequencies of 0.91 and 0.09, respectively, as well as availability of two genotypes - the homozygous wild GG and the heterozygous AG with frequencies of 0.84 and 0.16, respectively. No individuals with the homozygous mutant genotype AA were detected. A proven higher milk productivity was found in the ewes carriers of the homozygous genotype GG (126,989 l) compared to those with the heterozygous genotype AG (122,141 l). No significant differences in litter size were found between carriers of the two genotypes.
Keywords: Awassi sheep; FABP3 gene; genotype variation; litter size; milk yield
Citation: Dimitrova, I., Stancheva, N., Bozhilova-Sakova, M. & Tzonev, T. (2024). Effect of genotypic variation in the FABP3 gene on milk productivity and fertility in Awassi ewes. Bulg. J. Agric. Sci., 30 (Supplement 1), 47–52
| References: (click to open/close) | Ali, W., Ceyhan, A., Ali, M. & Dilawar, S. (2020). The merits of Awassi sheep and its milk along with major factors affecting its production. J. Food. Env. Sci., 1(1), 50-69.
Al-Samarai, F. & Al-Anbari, N. (2009). Genetic evaluation of rams for total milk yield in Iraqi Awassi sheep. J. agric. biol. sci., 4, 54-57.
Ayadi, M., Hayani, A., Matar, A., Salama, A., Abid, I. & Aljumaah, R. (2024). Influence of local breeds Najdi and Awassi on fatty acid composition and lipid quality of dairy sheep in Saudi Arabia. Journal of Animal and Plant Sciences, 34(4), 1091-1098. https://doi.org/10.36899/JAPS.2024.4.0791.
Bai, J., Xu, H., Zang, R., He, H., Cai, Y., Cao, X., Peng, F., Han, J., Wud, J. & Yangb, J. (2013). Cloning of the heart fatty acid-binding protein (H-FABP) gene and its tissue-specific expression profile in the Lanzhou fat-tailed sheep, Ovis aries. Small Ruminant Research, 112, 114– 122.
Bencini, R. & Pulina, G. (1997). The quality of sheep milk: a review. Australian Journal of Experimental Agriculture, 37, 485-504.
Boykovski, S., Stefanova, G. & Dimitrov, D. (2006). Breeds and offspring of sheep, raised in Bulgaria. Yuni Express, Shumen (Bg).
Boykovski, S., Georgiev, D., Hristov, M. & Tsochev, I. (2019). The Awassi sheep breed in Bulgaria. Himera, Shumen (Bg).
Calvo, J., Marcos, S., Jurando, J. & Serrano, M. (2004). Association of the heart fatty acid-binding protein (FABP3) gene with milk traits in Manchega breed sheep. Anim. Genet., 35, 347-349. DOI: 10.1111/j.1365-2052.2004.01169.x.
Calvo, J., Vaiman, D., Saïdi-Mehtar, N., Beattie, A., Jurando, J. & Serrano, M. (2002). Characterization, genetic variation and chromosomal assignment to sheep chromosome 2 of the ovine heart fatty acid-binding protein gene (FABP3). Cytogenet. Genome Res., 98, 270-273.
Chmurzynska, A. (2006). The multigene family of fatty acid-binding proteins (FABPs): function, structure and polymorphism. J. Appl. Genet., 47, 39–48.
Dimitrova, I., Bozhilova-Sakova, M., Ignatova, M. & Petrov, N. (2020). Polymorphism of FABP3 gene in some merino and local sheep breeds in Bulgaria. Comptes rendus de l’Acad´emie bulgare des Sciences, 73(5), 742-748. ISSN: 1310–1331 (Print), 2367–5535 (Online). DOI:10.7546/CRABS.2020.05.18.
Dimitrova, I., Bozhilova-Sakova, M. & Okyasheva. S. (2021a). Study on genetic diversity of genes FABP3 and GDF9 in Cooper-Red Shumen and Synthetic Population Bulgarian Milk sheep breed. Proceedings of 4th International Health Sciences and Innovation Congress, July 5-6, Baku, Azerbaijan, ISBN: 978-1-955094-10-8, 70 - 79.
Dimitrova, I., Bozhilova-Sakova, M., Ivanova, T., Koutev, V. & Ignatova, M. (2021b). Polymorphism of FABP3 gene and its effect on litter size and milk production of Synthetic Population Bulgarian milk ewes. Factors in Experimental Evolution of Organisms, 28, 48-52, UDC 575.17. DOI: https://doi.org/10.7124/FEEO.v28.1374.
Dimitrova, I., Bozhilova-Sakova, M. & Stancheva, N. (2022). Polymorphism identification of FABP3 gene in sheep of Bulgarian Dairy Synthetic Population. Scientific Papers. Series D. Animal Science, LXV(1), ISSN 2285-5750, 46-51.
Epstein, H. (1985). The Awassi sheep with special reference to the improved dairy type. Animal Production and Health Paper, 57(A), 282. Publ. by FAO, Rome.
Furuhashi, M. & Hotamisligil, G. S. (2008). Fatty acid-binding proteins: role in metabolic diseases and potential as drug targets. Nat. Rev., 7, 489–501.
Galal, S., Gürsoy, O. & Shaat, I. (2008). Awassi sheep as a genetic resource and efforts for their genetic improvement. A review. Small Ruminant Res., 79(2-3), 99-108.
Gootwine, E. (2011). Mini review: breeding Awassi and Assaf sheep for diverse management conditions. Trop. Anim. Health Prod., 43, 1289-1296. DOI: https://doi.org/10.1007/s11250-011-9852-y.
Gürsoy, O. (2011). Awassi and its possible rural development role in Africa and Asia. Macedonian J. Anim. Sci., 1, 305-316.
İnal, S., Coşkun, B., Dinç, D. & Çam, M. (2021). Reproductive parameters of primiparous Awassi sheep and mortality rates of their lambs in steppe climates J. Livestock Sci., 12, 262-267.
Javadi-Novashnagh, S., Moradi-Shahrbabak, M., Sadeghi, M., Ropka-Molik, K., Moradi-Shahrbabak, H. & Mura, M. (2015). Fatty Acid Binding Protein 3 Gene Polymorphisms and Their Associations with Growth Traits and Blood Parameters in Two Iranian Sheep Breeds. ICASVM: International Conference on Animal Science and Veterinary Medicine: June 04-05, 2015, New York, United States, v. 9 n 6.
Jawasreh, K. I., Haddad, N., Abu Lubad, A. & Al-Amareen, A. (2022). Genome-wide association study for milk production of Awassi sheep in Jordan, Journal of the Saudi Society of Agricultural Sciences, 21(5), 302-309. https://doi.org/10.1016/j.jssas.2021.09.006.
Jurie, C., Cassar-Malek, I., Bonnet, M., Leroux, C., Bauchart, D., Boulesteix, P., Pethick, D. W. & Hocquette, J. F. (2007). Adipocyte fatty acidbinding protein and mitochondrial enzyme activities in muscles as relevant indicators of marbling in cattle. J. Anim. Sci., 85, 2660–2669.
Kowalewska-Łuczak, I., Czerniawska-Piątkowska, E. & Pecka-Kiełb, E. (2017). Investigation on relationships of the FABP3 and SLC27A3 genes with milk production traits in sheep. Journal of Elementology, 22(4), 1485-1493.
Lanier, J. & Corl, B. (2015). Challenges in enriching milk fat with polyunsaturated fatty acids. Journal of Animal Science and Biotechnology, 6, 26.
Merzah, L., Mohammed, R., Rhadi, F. & Al-Thuwaini, T. (2023). Fatty Acid Synthase Polymorphism of Awassi Sheep and its Impact on Fatty Acid Composition. Fatty acid synthase polymorphism of Awassi sheep and its impact onfatty acid composition. JITV, 28(3), 152-158. DOI:http://dx.doi.org/10.14334/jitv.v28.i3.3169.
Meydan, H., Rashaydeh, F., Al-Atiyat, R. & Yildiz, B. (2024). Genetic diversity of Awassi sheep reared in Fertile crescent based on microsatellites: A review. Journal of Animal and Plant Sciences, 34(5). https://creativecommons.org/licenses/by/4.0/.
Stancheva, N., Dimitrova, I., Bozhilova-Sakova, M., Nenova, R. & Tzonev, T. (2023). Association of FABP3 gene polymorphism with litter size in ewes from the Bulgarian dairy synthetic population. Scientific Papers. Series D. Animal Science, LXV(1), 52-57.
Stancheva, N., Dimitrova, I., Bozhilova-Sakova, M. & Tzonev, T. (2024). Association of FABP3 gene polymorphism with milk production in ewes from the Bulgarian dairy synthetic population. Scientific Papers. Series D. Animal Science, LXVII(1), 74-79.
Üstüner, H. & Oğan, M. (2013). Main productive performance of Awassi sheep in the Central Anatolian Region of Turkey, Turkish Journal of Veterinary & Animal Sciences, 37(3), 271-276. https://doi.org/10.3906/vet-1205-13. |
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| Date published: 2024-12-13
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