Bulgarian Journal of Agricultural Science
Array ( [session_started] => 1730369673 [LEPTON_SESSION] => 1 [PAGE_ID] => 107 [LANGUAGE] => EN )
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Effectiveness of halotolerant diazotrophic bacteria in increasing nitrogen and potassium uptake of rice in high salinity medium
Purwanto, Eka Oktaviani, Zulfa Ulinuha, Tarjoko, Ni Wayan Anik Leana
Abstract: This study aimed to examine the effectiveness of indigenous diazotrophic bacteria from saline paddy fields of The Northern Coastal of Pemalang in increasing N and K nutrient uptake in rice plants. This research was conducted at the Experimental Farm and Agronomy & Horticulture Laboratory, Faculty of Agriculture, Jenderal Soedirman University, Purwokerto. This study was designed using a completely randomized block design with nitrogen fixing bacteria as the treatment and repeated three times. The treatment that was tried was a type of N2-fixing bacteria consisting of control without inoculation of bacteria (P0), Acinetobacter junii (P1), Bacillus tropicus (P2), Acinetobacter schindleri (P3), Pseudomonas stutzeri (P4), Bacillus altitudinis (P5), Bacillus cereus (P6), and Bacillus subtilis (P7). The study used the culture solution method with the source of nutrition from AB Mixtm nutrient with EC set at 5.5 dSm-1, and pH 5.5 – 6.5. Observation variables including N uptake by rice plants were analyzed at the maximum vegetative phase using the Kjedahl method, Na and K absorption of rice plants were analyzed by diacidic extraction method and the results were measured using a flame photometer. The research data were analyzed using ANOVA, and if they were significantly different, they continued with DMRT 5%. The result showed that inoculation of rice plants with Bacillus subtilis significantly increased plant biomass, N uptake, and K uptake. Diazotrophic bacteria inoculation was able to increase the N uptake of rice plants by an average of 0.341 g/plant (104.36%), while in the control treatment it was only 0.167 g/plant. Inoculation of diazotrophic bacteria in plants under salinity stress tends to reduce Na uptake in rice plants. Inoculation of diazotropic bacteria was able to increase the average K/Na ratio of 113.31%, and this indicated that inoculation of halotolerant diazotrophic bacteria was able to increase the tolerance of rice plants to salinity stress.
Keywords: bacteria; diazotropic; nutrient; rice; saline
Citation: Purwanto, Oktaviani, E., Ulinuha, Z., Tarjoko & Leana, N. W. A. (2024). Effectiveness of halotolerant diazotrophic bacteria in increasing nitrogen and potassium uptake of rice in high salinity medium. Bulg. J. Agric. Sci., 30(5),
888–892
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Date published: 2024-10-24
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