Effectiveness of halotolerant diazotrophic bacteria in increasing nitrogen and potassium uptake of rice in high salinity medium
Purwanto, Eka Oktaviani, Zulfa Ulinuha, Tarjoko, Ni Wayan Anik Leana
Abstract: This study aimed to examine the effectiveness of indigenous diazotrophic bacteria from saline paddy fields of The Northern Coastal of Pemalang in increasing N and K nutrient uptake in rice plants. This research was conducted at the Experimental Farm and Agronomy & Horticulture Laboratory, Faculty of Agriculture, Jenderal Soedirman University, Purwokerto. This study was designed using a completely randomized block design with nitrogen fixing bacteria as the treatment and repeated three times. The treatment that was tried was a type of N2-fixing bacteria consisting of control without inoculation of bacteria (P0), Acinetobacter junii (P1), Bacillus tropicus (P2), Acinetobacter schindleri (P3), Pseudomonas stutzeri (P4), Bacillus altitudinis (P5), Bacillus cereus (P6), and Bacillus subtilis (P7). The study used the culture solution method with the source of nutrition from AB Mixtm nutrient with EC set at 5.5 dSm-1, and pH 5.5 – 6.5. Observation variables including N uptake by rice plants were analyzed at the maximum vegetative phase using the Kjedahl method, Na and K absorption of rice plants were analyzed by diacidic extraction method and the results were measured using a flame photometer. The research data were analyzed using ANOVA, and if they were significantly different, they continued with DMRT 5%. The result showed that inoculation of rice plants with Bacillus subtilis significantly increased plant biomass, N uptake, and K uptake. Diazotrophic bacteria inoculation was able to increase the N uptake of rice plants by an average of 0.341 g/plant (104.36%), while in the control treatment it was only 0.167 g/plant. Inoculation of diazotrophic bacteria in plants under salinity stress tends to reduce Na uptake in rice plants. Inoculation of diazotropic bacteria was able to increase the average K/Na ratio of 113.31%, and this indicated that inoculation of halotolerant diazotrophic bacteria was able to increase the tolerance of rice plants to salinity stress.
Keywords: bacteria; diazotropic; nutrient; rice; saline
Citation: Purwanto, Oktaviani, E., Ulinuha, Z., Tarjoko & Leana, N. W. A. (2024). Effectiveness of halotolerant diazotrophic bacteria in increasing nitrogen and potassium uptake of rice in high salinity medium. Bulg. J. Agric. Sci., 30(5),
888–892
| References: (click to open/close) | Abd El-Rheem, M. & Zaki, S. S. (2018). Effect of soil salinity on growth, yield and nutrient balance of peanut plants. International Journal of ChemTech Research, 8(12), 564–568.
Basak, N., Rai, A. K., Sundha, P., Meena, R. L., Bedwal, S., Yadav, R. K. & Sharma, P. C. (2022). Assessing soil quality for rehabilitation of salt-affected agroecosystem : a comprehensive review. Front. Environ. Sci., 10, 935785, 1–15. https://doi.org/10.3389/fenvs.2022.935785
Erfandi, D. & Rachman, A. (2011). Identification of soil salinity due to seawater intrusion on rice field in the Northern Coast of Indramayu, West Java. J. Trop. Soils, 16(2), 115–121. https://doi.org/10.5400/jts.2011.16.2.115
Gao, Y., Zou, H., Wang, B. & Yuan, F. (2022). Progress and applications of plant growth-promoting bacteria in salt tolerance of crops. Int. J. Mol. Sci., 23(7036), 2–20.
Irshad, M., Eneji, A. E., Khattak, R. A. & Khan, A. (2009). Influence of nitrogen and saline water on the growth and partitioning of mineral content in maize. Journal of Plant Nutrition, 32, 37–41. https://doi.org/10.1080/01904160802660768
Jha, Y. & Subramanian, R. B. (2013). Paddy plants inoculated with PGPR show better growth physiology and nutrient content under saline conditions. Chilean Journal of Agricultural Research, 73(3), 213–219. https://doi.org/10.4067/S0718-58392013000300002
Junianti, E., Proklamasiningsih, E. & Purwanto. (2020). The effect of PGPR inoculation on the rice growth at vegetative phase. Jurnal Agro, 7(2), 193–202.
Kapadia, C., Sayyed, R. Z., Ali, H., Enshasy, E., Vaidya, H., Sharma, D., Patel, N., Malek, R. A., Syed, A., Elgorban, A. M., Ahmad, K., Tan, A. & Zuan, K. (2021). Halotolerant microbial consortia for sustainable mitigation of salinity stress, growth promotion, and mineral uptake in tomato plants and soil nutrient enrichment. Sustainability, 13(8369), 2–14.
Karolinoerita, V. & Annisa, W. (2020). Land salinization and its problems in Indonesia Jurnal Sumberdaya Lahan, 14(2), 91–99.
Khasanah, I. N. & Astuti, K. (2022). Harvest Area and Rice Production in Indonesia 2021.. Badan Pusat Statistik Indonesia.
Kumar, A., Singh, S., Gaurav, A. K., Srivastava, S. & Verma, J. P. (2020). Plant growth-promoting bacteria : biological tools for the mitigation of salinity stress in plants. Front. Microbiol., 11(1216), 1–15. https://doi.org/10.3389/fmicb.2020.01216
Machado, R. M. A. & Serralheiro, R. P. (2017). Soil Salinity: Effect on vegetable crop growth . management practices to prevent and mitigate soil salinization. Horticulturae, 3(30), 2–13. https://doi.org/10.3390/horticulturae3020030
Nawaz, A., Shahbaz, M., Asadullah, Imran, A., Marghoob, M.U., Imtiaz, M. & Mubeen, F. (2020). Potential of salt tolerant PGPR in growth and yield augmentation of wheat (Triticum aestivum L.) under saline conditions. Front. Microbiol., 11(2019), 1–12. https://doi.org/10.3389/fmicb.2020.02019
Paul, D. & Lade, H. (2015). Plant-growth-promoting rhizobacteria to improve crop growth in saline soils : a review. Agron. Sustain. Dev., 34(4), 737–752. https://doi.org/10.1007/s13593-014-0233-6
Pratama, A. R. & Harini, R. (2019). Analysis of the availability and demand for rice in Indonesia in 2018. Media Komunikas Geografi, 20(2), 101–114.
Purbiyanti, E., Yazid, M. & Januarti, I. (2017). The conversion of rice fields in Indonesia has an influence on the government's grain/rice purchasing price policy. Jurnal Manajemen Dan Agribisnis, 14(3), 209–217.
Reddy, I. N. B. L., Kim, S., Kim, B., Yoon, I. & Kwon, T. (2017). Identification of rice accessions associated with K+/Na+ ratio and salt tolerance based on physiological and molecular responses. Rice Science, 24(6), 360–364. https://doi.org/10.1016/j.rsci.2017.10.002
Shabaan, M., Asghar, H. N., Zahir, Z. A., Zhang, X., Sardar, M. F. & Li, H. (2022). Salt-tolerant PGPR confer salt tolerance to maize through enhanced soil biological health, enzymatic activities, nutrient uptake and antioxidant defense. Front. Microbiol., 13(901865), 1–13. https://doi.org/10.3389/fmicb.2022.901865
Shrivastava, P. & Kumar, R. (2015). Soil salinity: A serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi Journal of Biological Sciences, 22(2), 123–131. https://doi.org/10.1016/j.sjbs.2014.12.001
Shultana, R., Tan, A., Zuan, K., Yusop, M. R. & Saud, H. M. (2020a). Characterization of salt-tolerant plant growth- promoting rhizobacteria and the effect on growth and yield of saline-affected rice. Plos One, 15(9), 1–16. https://doi.org/10.1371/journal.pone.0238537
Shultana, R., Zuan, A. T. K., Yusop, M. R., Saud, H. M. & Ayanda, A. F. (2020b). Effect of salt-tolerant bacterial inoculations on rice seedlings differing in salt-tolerance under saline. Agronomy, 10(1030), 2–22.
Syarifuddin, M., Nuraini, I. & Wahyudi, S. (2019). Analysis of rice production for each province in Indonesia 2011-2016. Jurnal Ilmu Ekonomi (JIE), 3(4), 517–531.
Tedeschi, A. (2020). Irrigated Agriculture on saline soils: a perspective. Agronomy, 10(1630), 4–9.
Upadhyay, S. K. & Singh, D. P. (2014). Effect of salt-tolerant plant growth-promoting rhizobacteria on wheat plants and soil health in a saline environment. Plant Biology, 17(1), 288–293. https://doi.org/10.1111/plb.12173
Wardani, C., Jamhari, Hardyastuti, S. & Suryantini, A. (2019). Rice resilience performance in Indonesia: comparison of Java and Outside Java for the 2005-2017 period. Jurnal Ketahanan Nasional, 25(1), 107–130.
|
|
| Date published: 2024-10-24
Download full text 
