Application of fluorescence spectroscopy in the determination of varietal differences after potato harvesting
Emilia Nacheva, Vanya Slavova
Abstract: The present study aims to establish the application of fluorescence spectroscopy as a field method in the determination of varietal differences after potato harvesting.
The experimental studies were conducted on site at the Institute of Vegetable Crops "Maritsa" after potato harvesting in 2021. The fluorescence analysis was carried out with a source with an emission wavelength of 285 nm and a portable fiber-optical spectrometer model, AvaSpec- ULS2048CL-EVO.
The subject of this research are tubers from Tresor (early variety standard), Sante (medium early variety standard), Agria (medium late variety standard), C 617 (early breeding line), D 486 (early breeding line), C 619 (medium early breeding line), B 782 (medium early breeding line), B 783 (medium-late breeding line), C 716 (medium late breeding line).
The correlation between their emission signals of the samples was established. This fact allows fluorescence spectroscopy to be successfully applied as a rapid tool to establish the origin of unknown tubers in the presence of a rich library of spectra as an applied tool in breeding programs.
The results of the experiment can be used to optimize the time for the analysis of the varietal difference of the potato genotypes after harvest.
Fluorescence spectroscopy in a fiber-optical configuration will support the process of determining the belonging of a specific variety to a given variety (even for samples of unknown origin, when it is necessary to qualify the result of аccessions in a short time.
The experimental studies were conducted on site at the Institute of Vegetable Crops "Maritsa" after potato harvesting in 2021. The fluorescence analysis was carried out with a source with an emission wavelength of 285 nm and a portable fiber-optical spectrometer model, AvaSpec- ULS2048CL-EVO.
The subject of this research are tubers from Tresor (early variety standard), Sante (medium early variety standard), Agria (medium late variety standard), C 617 (early breeding line), D 486 (early breeding line), C 619 (medium early breeding line), B 782 (medium early breeding line), B 783 (medium-late breeding line), C 716 (medium late breeding line).
The correlation between their emission signals of the samples was established. This fact allows fluorescence spectroscopy to be successfully applied as a rapid tool to establish the origin of unknown tubers in the presence of a rich library of spectra as an applied tool in breeding programs.
The results of the experiment can be used to optimize the time for the analysis of the varietal difference of the potato genotypes after harvest.
Fluorescence spectroscopy in a fiber-optical configuration will support the process of determining the belonging of a specific variety to a given variety (even for samples of unknown origin, when it is necessary to qualify the result of аccessions in a short time.
Keywords: breeding line; emission wavelength; fluorescence spectroscopy; potato tubers; variety
Citation: Nacheva. E. & Slavova, V. (2024). Application of fluorescence spectroscopy in the determination of varietal differences after potato harvesting. Bulg. J. Agric. Sci., 30(2), 305–309
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Date published: 2024-04-26
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