Soil Bacillus spp. a potent cell factory for antimicrobials against phytopathogens
Natalija A. - Pancevska
Abstract: Many plant diseases are caused by phytopathogenic bacteria which greatly determine the quality of plant production. The biological control of plant pathogenic bacteria is an alternative method to the application of chemicals, which may be accomplished through the destruction of existing inoculums, exclusion from the host, or the suppression or displacement of the pathogen after infection. It offers an environmentally friendly approach to the management of plant disease and can be incorporated with cultural and physical control and limited chemical usage for an effective and integrated disease-management system. Biological control includes the use of beneficial microorganisms, such as specialized fungi and bacteria, to attack and control plant pathogens and the diseases they cause. Biological control is an innovative, cost effective and eco-friendly approach for control of many plant diseases.
Bacteria of the genus Bacillus has showed antimicrobial activity against plant pathogenic microorganisms. Bacillus spp. are natural inhabitants of the phyllosphere and rhizosphere. These bacteria are involved in the control of plant diseases through a variety of mechanisms of action, such as competition, systemic resistance induction and antibiotic production. The mechanism of antibiosis has been shown to be one of the most important. Bacillus spp. have the advantage of being already adapted to the environment where they can be applied as bio-logical control. They have the characteristics of having high thermal tolerance, showing rapid growth in liquid culture, and readily form resistant spores. It is considered safe biological agents and their potential as biocontrol agents is considered to be high.
This research is based on the isolation and screening of biocontrol activities of soil Bacillus sp. against several phytopathogens in in vitro study. Since tested isolate has showed the production of antimicrobials against the growth of selected phytopathogens, in further work, all of this trial need to be supported by evaluation of antimicrobial activity in in vivo.
Keywords: antimicrobial effects; Bacillus spp.; biocontrol; in vitro study; phytopathogens
Citation: Atanasova-Pancevska, N. (2024). Soil Bacillus spp. – a potent cell factory for antimicrobials against phytopathogens. Bulg. J. Agric. Sci., 30(2), 219–227
| References: (click to open/close) | Agarwal, M., Dheeman, S., Dubey, R. C., Kumar, P., Maheshwari, D. K. & Bajpai, V. K. (2017). Differential antagonistic responses of Bacillus pumilus MSUA3 against Rhizoctonia solani and Fusarium oxysporum causing fungal diseases in Fagopyrum esculentum Moench. Microbiol. Res., 205(9), 40–47.
Agrios, G. N. (2005). Plant Pathology. 5th Edition: Elsevier Academic Press. Burlington, Ma. USA 79–103.
Cazorla, F. M., Romero, D., Pérez-García, A., Lugtenberg, B. J. J., Vicente, A. D. & Bloemberg, G. (2007). Isolation and characterization of antagonistic Bacillus subtilis strains from the avocado rhizoplane displaying biocontrol activity. J. Appl. Microbiol., 103(5), 1950–1959.
Chisholm, S. T., Coaker, G., Day, B. & Staskawicz, B. J. (2006). Host-microbe interactions: Shaping the evolution of the plant immune response. Cell, 124, 803 − 814.
Compant, S., Reiter, B., Sessitsch, A., Nowak, J., Clément, C., Ait Barka, E. (2005). Endophytic colonization of Vitis vinifera L. by a plant growth-promoting bacterium, Burkholderia sp. strain PsJN. Appl. Environ. Microbiol. 71, 1685-1693.
Davis, E. L., Hussey, R. S., Mitchum, M. G. & Baum, T. J. (2008). Parasitism proteins in nematode–plant interactions. Current Opinion in Plant Biology, 11, 360–366.
Edwards, S., McKay, T. & Seddon, B. (1994). Interaction of Bacillus species with phytopathogenic fungi. Methods of analysis and manipulation for biocontrol purposes. Ecology of Plant Pathogens, 101-118.
Hossain, M. N. & Rahman, M. M. (2014). Antagonistic activity of antibiotic producing Streptomyces sp. against fish and human pathogenic bacteria. Brazilian Arch. Biol. Technol., 57(2), 233–237.
Layton, C., Maldonado, E., Monroy, L., Corrales, L. C. & Sánchez, L. C. (2011). Bacillus spp.; perspective biocontrol through antibiosis effect on crops affected by phytopathogenics. Revista NOVA Publicación Científica en Ciencias Biomédicas, 9, 177-187.
Lee, T., Park, D., Kim, K., Lim, S. M., Yu, N. H. & Kim, S. (2017). Characterization of Bacillus amyloliquefaciens DA12 showing potent antifungal activity against mycotoxigenic fusarium species. Plant Pathol. J., 33(5), 499–507.
Li, J., Yang, Q. Z. L., Zhang, S. W. Y. & Zhao, X. (2009). Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29. J. Zhejiang Univ. Sci., B 10(4), 264–272.
Maas, J. L. (2004). Strawberry disease management. In: Diseases of Fruits and Vegetables, Kluwer Academic Publishers, Netherlands, II, 441-483.
Madhaiyan, M., Poonguzhali, S., Kwon, S.-W. & Sa, T.-M. (2010). Bacillus methylotrophicus sp. nov., a methanol-utilizing, plant-growth-promoting bacterium isolated from rice rhizosphere soil. International Journal of Systematic and Evolutionary Microbiology, 60(10), 2490-2495.
Massart, S. & Jijakli, H. M. (2007). Use of molecular techniques to elucidate the mechanisms of action of fungal biocontrol agents: A review. Journal of Microbiological Methods, 69, 229-241.
Nicholson, W. L., Munakata, N., Horneck, G. & Melosh, H. J. S. P. (2000). Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiol. Mol. Biol. Rev., 64(3), 548–572.
Pal, K. & Gardener, B. (2006). The Plant Health Instructor in Biological Control of Plant Pathogens. Constable, London, 206.
Raaijmakers, J. M. & Mazzola, M. (2012). Diversity and Natural Functions of Antibiotics Produced by Beneficial and Plant Pathogenic Bacteria. Annual Reviews of Phytopathology, 50, 403-424.
Romero, D., García, A. P., Rivera, M. E., Cazorla, F. M. & Vicente, A. (2004). Isolation and evaluation of antagonistic bacteria towards the cucurbit powdery mildew fungus Podosphaera fusca. Appl. Microbiol. Biotechnol., 64(2), 263–269.
Romero, D., de Vicente, A., Rakotoaly, R. H., Dufour, S. E., Veening, J.-W., Arrebola, E. & Pérez-García, A. (2007). The iturin and fengycin families of lipopeptides are key factors in antagonism of Bacillus subtilis toward Podosphaera fusca. Molecular Plant-Microbe Interactions, 20(4), 430-440.
Saha, D., Purkayastha, G. D., Ghosh, A. & Isha, M. S. A. (2012). Isolation and characterization of two new Bacillus subtilis strains from the rhizosphere of eggplant as potential biocontrol agents. J. Plant Pathol., 94(1), 109–118.
Santoyo, G., del Orozco-Mosqueda, M. C. & Govindappa, M. (2012). Mechanisms of biocontrol and plant growth-promoting activity in soil bacterial species of Bacillus and Pseudomonas: a review. Biocontrol Sci. Technol., 22(8), 855–872.
Shrestha, B. K., Karki, H. S., Groth, D. E., Jungkhun, N. & Ham, J. H. (2016). Biological control activities of rice-associated Bacillus sp. strains against sheath blight and bacterial panicle blight of rice. PLoS ONE, 11(1), 1–18.
Snn, P., Cui, J., Jia, X. & Wang, W. (2017). Isolation and characterization of Bacillus amyloliquefaciens L-1 for biocontrol of pear ring rot. Hortic. Plant J., 3(5), 183–189.
Strange, R. N. & Scott, P. R. (2005). Plant disease: a threat to global food security. Annu. Rev. Phytopathol., 43, 83–116.
Tejera-Hernández, B., Rojas-Badía, M. M. & Heydrich-Pérez, M. (2011). Potential of the Bacillus genus in promoting plant growth and biological control of phytopathogenic fungi. Revista CENIC Ciencias Biológicas, 42, 131-138.
Wang, H., Wen, K., Zhao, X., Wang, X., Li, A. & Hong, H. (2009). The inhibitory activity of endophytic Bacillus sp. strain CHM1 against plant pathogenic fungi and its plant growth-promoting effect, Crop Protection, 28(8), 634-639.
Wulff, E. G., Mguni, C. M., Mansfeld-Giese, K., Fels, J., Lübeck, M. & Hockenhull, J. (2002). Biochemical and molecular characterization of Bacillus amyloliquefaciens, B. subtilis and B. pumilus isolates with distinct antagonistic potential against Xanthomonas campestris pv. campestris. Plant Pathol., 51(5), 574–584.
Yoshida, S., Hiradate, S., Tsukamat, T., Hatakeda, K. & Shirata, A. (2001). Antimicrobial activity of culture filtrate Bacillus amyloliquefaciens RC-2 isolated from mulberry leaves. Phytopathology, 91, 181-187.
Zhang, Q.-L., Liu, Y., Ai, G.-M., Miao, L.-L., Zheng, H.-Y. & Liu, Z.-P. (2012). The characteristics of a novel heterotrophic nitrification–aerobic denitrification bacterium, Bacillus methylotrophicus strain L7. Bioresource Technology, 108, 35-44.
|
|
| Date published: 2024-04-26
Download full text 
