Salinity effect on seed germination in doubled haploid and parental barley genotypes (Hordeum vulgare L.)

Samira Hentour, Rabouan Oubaidou, Younes El Goumi and Malika Fakiri

Abstract: In cereals, resistance and tolerance to salinity are qualities that allow their cultivation in arid and semi-arid regions. Hence, we sought to select salt stress-resistant barley genotypes by applying sub-lethal NaCl concentrations and observing their effect on germination. This work was carried out using parent spring barley genotype (Hordeum vulagre L.) namely Tamelalt, and its doubled haploids progeny. Salt stress resistance was tested on seeds germination in Petri dishes provided with filter paper containing increasing concentrations of salt (0, 5, 10, and 15 g.L-1) for 10 days at 25°C. The doubled haploids progeny derived from Tamelalt showed to be the most tolerant to salt stress with a germination rate under the most severe salt stress conditions (NaCl 15 g.L-1), while the parent genotype Tamelalt was the least tolerant with a germination rate of only 26.67% under the same stress. Principal component analysis and hierarchical cluster analysis allowed the separation of DH lines and parent genotype. Two groups were identified. The DH lines were completely different from the parent genotype.

Keywords: barley; doubled haploid lines; germination; Hordeum vulagre L.; salinity

Date published: 2022-10-24