Effect of three extenders on the motility and morphological characteristics of spermatozoa in diluted Muscovy semen stored at 4C up to 120 hours

Vasko Gerzilov and Madlena Andreeva

Abstract: The present study evaluated the total and progressive motility, as well as sperm morphological characteristics of diluted semen with three different extenders (1:3 v/v) and stored at 4°C for 3, 6, 24, 30, 48, 54, 72, 78, 96, 120 h. Total (TSM) and progressive mobility (PSM) gradually decrease with prolonging storage time. Up to the 30 h of sperm storage the sperm maintain high TSM – over 80% in semen diluted with IMV Canadyl and AU extenders and over 68% when diluted with HIA-1 extenders. With increasing in vitro sperm storage time, PSM and rapid sperm cells decrease significantly after the 3rd h, while non-progressive motility increases until the 48th h, after which it also begins to decrease. For velocity parameters VCL, VAP, VSL are reported a more rapid trend of decrease between 3 and 30 h of cold storage, after which the decline is smoother (P<0.05). As storage time is extended, the pathological forms of the sperm cells increase, with those affecting the head of the sperm cells predominating – crooked-neck and swelling forms.

Keywords: extender; liquid storage; Muscovy duck; semen; sperm morphology; sperm motility

Date published: 2021-12-16