In vitro propagation of the Balkan endemic species Stachys leucoglossa Griseb.

Veneta M. Kapchina, Zhenya P. Yordanova, Desislava I. Mantovska

Abstract: In vitro shoot culture of the Balkan endemic species Stachys leucoglossa Griseb. was initiated by successful sterilization of seeds with 70% ethanol. Regenerated plants on hormone free MS medium had low growth index, shortened internodes and did not form a root system. Then the effect of different concentrations of cytokinine 6-benzylaminopourine (BA) and auxins – indole-3-butyric acid (IBA) and naphthyl acetic acid (NAA) on the in vitro multiplication of S. leucoglossa was studied. MS medium supplemented with 0.5 mg/L BA was most effective for shoot development and approximately 75% of the explants showed shoot proliferation and produced 5.61Ѓ}1.15 shoots per explant. For stimulating root development in vitro cultivated S. leucoglossa plants were cultivated on WPM medium with different concentrations of IBA and NAA. Root formation was observed in 60% of the explants cultivated on WPM media supplemented with 0.5 mg/L IBA and 40% with WPM media supplemented with 0.5 mg/L NAA. A collection of in vitro tissue culture was successfully established, which is an alternative approach for preservation of S. leucoglossa Griseb.

Keywords: auxin; cytokinin; in vitro multiplication; shoot culture; woundwort

Date published: 2019-12-19