Induction of total phenol and salicylic acid with Pseudomonas spp. suspension as indications increasing systemic resistance on vanilla plant

Ni Putu Pandawani, I. Ketut Widnyana, Putu Eka Pasmidi Ariati, I. Ketut Sumantra

Abstract: This study aims to determine the effect of Pseudomonas spp. in inducing the resistance of vanilla plants characterized by increasing total phenol content and salicylic acid in vanilla plant tissue after inoculation with Pseudomonas spp. suspension. In this study, vanilla cuttings were inoculated with Pseudomonas spp. suspension with a population density of 5 x106 CFU applied by root immersion, injection of the stem, and injection of vanilla leaves. The results showed that there was an increase of total phenol and salicylic acid content in vanilla plant tissue. The highest total phenol content was found in the Pseudomonas spp. W80 suspension treatment, which averaged 260.27 mg/g or an increase 106.89% compared to the distillated water treatment as control. The total phenol content average in the 2.6.D (2.6-dichloro-isonicotinic acid (INA) treatment is 240.80 mg/g or an increase of 91.41% compared to control. The highest salicylic acid content found in the treatment of Pseudomonas spp. W02 suspension is 37.65 ppm or 72.94% increase compared to control, followed by 2.6.D treatment of 32.55 ppm or 49.52% increase compared to control. Bacteria Pseudomonas spp. W02, W68, and W80 isolates has the ability to induce vanilla plant resistance with different abilities.

Keywords: induction; Pseudomonas spp.; resistance; salicylic acid; total phenol; vanilla

Date published: 2019-07-31