EFFECT OF PLANT GROWTH REGULATORS ON CHLOROPLAST ULTRA STRUCTURE IN LAMIUM ALBUM PLANTLETS

D. Koleva, T. Ganeva, M. Stefanova

Abstract: Lamium album L. stem nodal segments were grown on Murashige and Skoog’s medium without growth regulators (control) and on media supplemented with 0.2 mg l-1 N6-benzyladenine, 0.5 mg l-1 indole-3-butyric acid or both plant growth regulators. The plastid apparatus of in vitro formed leaflets was studied by transmission electron microscopy. The cytokinin N6-benzyladenine negatively affected the chloroplast formation when was applied individually or in combination with the auxin indole-3-butyric acid. On the contrary, IBA in concentration of 0.5 mg l-1 was appropriate for development of chloroplasts with regular shape and well organized internal membrane system thus providing for the regeneration capacity in vitro.

Keywords: in vitro-regeneration; indole-3-butyric acid; medicinal plant; N6 benzyladenine; transmission electron microscopy; white dead nettle

Date published: 2019-01-17