Isolation and characterization of biosurfactant-producing bacteria isolated from agriculture area in Thailand

Pannapa Powthong, Pattra Suntornthiticharoen

Abstract: Biosurfactant-producing bacteria were isolated from various soil and water in agricultural area of Thailand. Based on hemolytic activity, drop collapsing test, oil displacement test, as well as emulsifi cation activity (%EA), 8 effective isolates from 234 isolates were selected. All selected isolates were tested for antimicrobial activities against plant pathogenic bacteria. It was
revealed that all isolates can inhibit more than 2 tested-plant pathogenic bacteria. The crude extracts of selected isolates were tested for stability of pH, NaCl, CaCl2, MgCl2 and temperature by measuring %EA and showed stability over a broad range of these conditions. All interested isolates were identifi ed by biochemical test and MALDI-TOF MS. The isolates were: 1 isolate Alcaligenes faecalis subsp. faecalis, 2 isolates Proteus mirabilis, and 1 isolate Providencia alcalifaciens. Overall, the strains found in this study were useful for the bioremediation of the agricultural area.

Keywords: agricultural area; antimicrobial activity; biodegradation; biosurfactant

Date published: 2018-08-30