Bulgarian Journal of Agricultural Science
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ASSESSMENT OF GENETIC POLYMORPHISM IN DURUM AND BREAD WHEAT ACCESSIONS OF AZERBAIJAN
Ram. Sharma, Sevinj Ahmadova, Vusala Izzatullayeva, Mehraj Abbasov, Zeynal Akparov, Shafag Mammadova, Sabina Hajiyeva, Sevda Babayeva, Ramiz Aliyev, Elchin Hajiyev
Abstract: The present paper aims at analyzing the genetic diversity among 110 bread and 110 durum wheat accessions and local varieties using inter simple sequence repeat (ISSR) markers. Eight ISSR primers generated a total of 107 and 136 PCR fragments for durum and bread wheat genotypes, respectively. In average ISSR primers produced 17 bands for bread and 13.3 bands for durum wheat genotypes. The level of genetic diversity (GDI) was quite high for both wheat species and averaged 0.94 for durum and 0.91 for bread wheat accessions. Results of cluster analysis of hard and soft wheat enabled to allocate 11 and 12 main clusters, respectively and revealed the complex nature of the distribution of the studied varieties and botanical varieties. There was no particular relation between genetic structure of genotypes and their geographical distribution. The most remote genotypes identified as a result of cluster analysis can be recommended for use by breeders to produce genetically diverse populations with a maximum range of variation in the hybrid offspring.
Keywords: bread wheat; durum wheat; genetic diversity; ISSR markers
Date published: 2018-03-07
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