CHARACTERISATION OF SIX ISOLATED BACTERIAL STRAINS FROM GENUS BACILLUS APPLICABLE IN ORGANIC FARMING

Y. Evstatieva, M. Badalova, V. Savov, D. Nikolova, Ts. Licheva

Abstract: The aim of this study was to determine the activity of six isolated strains from genus Bacillus applicable in organic farming. These strains were cultivated in two different liquid nutrient media in order to examine their growth. Monitoring of the growth was carried out in order to achieve high concentrations of active cells. The results showed that the better medium for achieving high concentrations of active cells for all tested strains was PDG. The enzymatic potential of six isolated strains was assessed by the API ZYM system (BioMérieux, France) by semi-quantitative scale from 0 to 5. The strains showed phosphatase (acid and alkaline) and phospho-hydrolase activities in the range 4–5. For confirmation of the results qualitative analysis for determination
of phosphatase activity by the method of Pikovska was performed. The effect of the strains on the growth of test plant Pisumsativum was studied (in vivo). The results showed that when Pisumsativum was inoculated with strains T 3, T 10 and T 18, the fresh root biomass and fresh shoot biomass increased signifi cantly.

Keywords: Bacillus; phosphatase activity; Pisumsativum

Date published: 2017-10-19