POLYMORPHISMS IN OBESITY-RELATED LEPTIN GENE AND ITS ASSOCIATION WITH REPRODUCTIVE TRAITS OF SOWS

Lyubov Getmantseva, Nekruz Bakoev, Vyacheslav Vaselenko, Aleksander Usatov, Siroj Bakoev, Anatoly Kolosov, Maxim Makarenko, Anastasia Radyuk, Aleksander Klimenko, Maria Leonova

Abstract: Leptin and other hormones secreted by fatty tissue increasingly draw attention as mammalian reproduction markers. In this paper, we have studied the effect of c.3469 T <C SNP of the LEP gene on Large White sows reproductive traits according to all litters and differentially according to each litters (1st, 2nd and 3rd litter). Research was conducted on purebred pigs of Large White (♀n = 354) bread in Russia. The productivity of pigs was evaluated according to the following traits: Total Number of piglets Born (TNB), Number of piglets Born Alive (NBA), Weight of Litter (WL). The c.3469 T<C SNP of LEP was determined by PCR-RFLP method. Analysis of the LEP gene polymorphism effect on the reproductive traits was carried out using mixed linear models. The data obtained for all litters show significant differences in the reproductive traits of pigs
specifi ed by c.3469 T<C SNP of LEP. The best reproductive traits are associated with the genotype CC. Sows with genotype CC exceeded their analogues with genotype TT by TNB 1.3 ± 0.5 (p = 0.04), NBA 1.33 ± 0.5 (p = 0.03) and WL 3.2 ± 1.01 (p = 0.001). The effect of polymorphism of the LEP on TNB and NBA may depend on the litter’s number. In our studies, with a differentiated estimate for the 1st, 2nd, and 3rd litter, the statistically significance effect by c.3469 T<C SNP of LEP on the TNB and NBA was determined only for the third litter. Further studies are needed to clarify the role of LEP polymorphism and other adipose tissue hormones and their receptors on the reproductive traits of pigs and opportunities to use them as SNP markers in breeding programs.

Keywords: leptin; marker; pig; reproductive traits; SNP

Date published: 2017-10-18