ANTIOXIDANT AND ANTICANCER ACTIVITIES OF MISTLETOE TEA PREPARED BY HIGH TEMPERATURE EXTRACTION WITH CYCLODEXTRIN

De-Xing Hou, S. I. Rahmawati, N. Hayashi, K. Ishimaru

Abstract: Indonesian tea mistletoe (Scurrulla atropurpurea, BL.) is a medicinal plant that can be used to treat cancer. In our previous study, mistletoe extract that have the greatest functionality was obtained by a high temperature batch extraction (HT BE) with 30% ethanol solvent at temperature 100˚C for 10 minutes under 0.2 MPa pressurize. To optimize the extract solution by extracts other compounds that have high activities but poorly soluble in aqueous solution, we added hydroxypropyl-b-cyclodextrin (b-CD) to the extraction solvent. This study was, especially, to investigate how the addition of b-CD to the extraction influenced the yield of the extract, the antioxidant activities and the total phenolic content of the extract. The extracts were also evaluated for their cytotoxic effects in colon cancer cells and normal cells. The assays showed significant difference for all the treatments, which demonstrate that the addition of b-CD influenced the bioactivities of the extracts. Furthermore, the addition of b-CD extract resulted effective to inhibit the growth of cancer cells.

Keywords: anticancer; antioxidant; cyclodextrin; high temperature extraction; mistletoe

Date published: 2017-10-16