Factors affecting chloride concentration in tobacco: A review
Radka Bozhinova

, Yovko Dyulgerski, Violeta Nikolova, Nikolay Nikolov
Abstract: Chloride is an essential micronutrient for tobacco plants. Small amounts of Cl– can improve tobacco growth and yield. It is generally accepted that concentrations in excess of 1% can produce poor-quality tobacco. The significant sources of chloride for tobacco plants are soil, Cl-containing fertilizers, and irrigation water. Responses of tobacco to chloride vary according to tobacco types and cultivars. The influence of chloride on the growth, yield, and chemical composition is inconsistent. In general, leaf chloride and reducing sugar concentrations increase with increasing soil or irrigation water chloride levels. Application rates of chloride-containing fertilizers or chloride levels in irrigation water should be monitored to prevent excessive chloride accumulation in tobacco leaves. Plant genetics affect chloride accumulation; therefore, cultivar selection is important in achieving lower chloride concentration in tobacco.
Keywords: chloride; fertilization; genotype; irrigation water; soil; tobacco
Citation: Bozhinova, R., Dyulgerski, Yo., Nikolova, V. & Nikolov, N. (2026). Factors affecting chloride concentration in tobacco: A review. Bulg. J. Agric. Sci., 32(1), 77–84
| References: (click to open/close) | Afzal, S., Chaudhary, N. & Singh, N. K. (2021). Role of Soluble Sugars in Metabolism and Sensing Under Abiotic Stress. In: Plant Growth Regulators. Editors: Aftab, T. and Hakeem, K. R., Berlin/Heidelberg, Germany, Springer, 305 - 334. Basirnia, A., Maleki, H., Darvishzadeh, R. & Ghavami, F. (2014). Mixed linear model association mapping for low chloride accumulation rate in oriental-type tobacco (Nicotiana tabaccum L.) germplasm. Journal of Plant Interactions, 9(1), 666 - 672. Bozhinova, R. (2012). Investigation of Chloride Concentration in Burley Tobacco Varieties. Tobacco, 62(7-12), 103 - 108. Cadersa, Y. & Atawoo, A. (2002). Assessment of the chloride status in the tobacco leaf and some potential sources for the high chloride level. In: Proceedings of the Fifth Annual Meeting of Agricultural Scientists, Réduit, Mauritius, 3 - 4 May 2001, Editors: Lalouette, J. A. and Bachraz, D. Y., 65 - 71. Çakir, R. & Çebi, U. (2010). The effect of irrigation scheduling and water stress on the maturity and chemical composition of Virginia tobacco leaf. Field Crops Research, 119(2-3), 269 - 276. Chen, W., He, Z. L., Yang, X. E., Mishra, S. & Stoffella, P. (2010). Chloride nutrition of higher plants: Progress and perspectives. Journal of Plant Nutrition, 33(7), 943 - 952. Chouteau, J. (1968). Effect of farmyard manure applications on chloride content and burning quality of tobaccos. SEITA Annls, 2(5), 25 - 37. Colmenero-Flores, J. M., Franco-Navarro, J. D., Cubero-Font, P., Peinado-Torrubia, P. & Rosales, M. A. (2019). Chloride as a Beneficial Macronutrient in Higher Plants: New Roles and Regulation. International Journal of Molecular Sciences, 20(19), 4686. Darvishzadeh, R. & Alavi, R. (2011). Genetic analysis of chloride concentration in oriental tobacco genotypes. Journal of Plant Nutrition, 34(7), 1070 - 1078. Darvishzadeh, R., Alavi, S. R. & Sarafi, A. (2011). Genetic variability for chloride concentration in oriental tobacco genotypes. Archives of Agronomy and Soil Science, 57(2), 167 - 177. Fan, Y. & Zhang, X. (2004). Study on chloride's main origins and accumulation in flue-cured tobacco. In: CORESTA Congress, 3-7 October 2004, Kyoto, Japan (Abstracts), A 10. Flower, K. C. (1999). Agronomy and Physiology, Field Practices. In: Tobacco Production, Chemistry and Technology. Editors: Davis, D. and Nielsen, M., Blackwell Science, Cambridge, 104 - 142. Franco-Navarro, J. D., Brumós, J., Rosales, M. A., Cubero-Font, P., Talón, M. & Colmenero-Flores, J. M. (2016). Chloride regulates leaf cell size and water relations in tobacco plants. Journal of experimental botany, 67(3), 873 - 891. Fuqua, B. D., Sims, J. L., Leggett, J. E., Benner, J. F. & Atkinson, W. O. (1976). Nitrate and chloride fertilization effects on yield and chemical composition of burley tobacco leaves and smoke. Canadian Journal of Plant Science, 56(4), 893 - 899. Geilfus, C. M. (2018). Review on the significance of chloride for crop yield and quality. Plant Science, 270, 114 - 122. Geilfus, C. M. (2019). Chloride in soil: From nutrient to soil pollutant. Environmental and Experimental Botany, 157, 299 - 309. Gul, H., Khattak, R. & Muhammad, D. (2006). Yield and chemical composition of tobacco leaves of different cultivars as affected by four levels of potassium chloride. Pakistan Journal of Scientific and Industrial Research, 49, 125 - 133. Guo, F., Liu, Q., Yan, X., Gao, Y., Han, H., Zu, J. & Wu, Z. (2019). Effect of irrigation water chloride content and irrigation times on chloride content of flue-cured tobacco leaves. Chinese Agricultural Science Bulletin, 35(32), 31 - 35 (Chinese). Ishizaki, H. & Akiya, T. (1978). Effects of chloride on growth and quality of tobacco. Jarq-japan Agricultural Research Quarterly, 12, 1 - 6. Johnson, G. D. & Sims, J. L. (1986). Response of burley tobacco to application date, source, and rate of potassium fertilizer. Tobacco Science, 30-36, 138 - 141. Karaivazoglou, N. A., Papakosta, D. K. & Divanidis, S. (2005). Effect of chloride in irrigation water and form of nitrogen fertilizer on Virginia (flue-cured) tobacco. Field Crops Research, 92(1), 61 - 74. Karaivazoglou, N. A., Papakosta, D. K. & Divanidis, S. (2006). Effect of Chloride in Irrigation Water on Oriental (Sun-Cured) Tobacco. Journal of Plant Nutrition, 29(8), 1413 - 1431. Keeney, A., Pearce, R. & Bailey, W. (2021). Effect of Potassium Source and Rate on Yield, Quality, and Tobacco-Specific Nitrosamines in Dark and Burley Tobacco. Tobacco Science, 58(1), 1 - 8. Kitamura, T., Ito, T., Kudo, H. & Ohzeki, K. (1978). Studies on the absorption of chloride by Burley tobacco. 1. Effects of chloride content in cured leaves on the chemical and physical properties and smoking quality of tobacco. Bulletin of the Morioka Tobacco Experiment Station, 13, 1 - 12. Krishnamurthy, V. & Nagarajan, K. (2001). A manual on soil testing and irrigation water analysis for tobacco. Central Tobacco Research Institute, Rajahmundry, 1 - 65. Langeroodi, A. R. S., Ghooshchi, F. & Dadgar, T. (2017). Alleviatory activities in mycorrhizal tobacco plants subjected to increasing chloride in irrigation water. Italian Journal of Agronomy, 12(1), 8 - 16. McCants, C. B. & Woltz, W. G. (1967) Growth and Mineral Nutrition of Tobacco. Advances in Agronomy, 19, 211 - 265. Metochis, C. & Orphanos, P. I. (1990). Course of chloride concentration in tobacco leaves through the harvesting season. Journal of Plant Nutrition, 13(5), 485 - 493. Mulchi, C. L. (1982). Chloride effects on agronomic, chemical and physical properties of Maryland tobacco – I. Response to chloride applied to the soil. Tobacco Science, 26, 113 - 116. Nemati, I., Moradi, F., Gholizadeh, S., Esmaeili, M. A. & Bihamta, M. R. (2011). The effect of salinity stress on ions and soluble sugars distribution in leaves, leaf sheaths and roots of rice (Oryza sativa L.) seedlings. Plant, Soil and Environment, 57(1), 26 - 33. Pace, C., Vann, M., Fisher, L. & Hardy, D. (2020). Implications of chloride application rate and nitrogen fertilizer source to flue-cured tobacco. Agronomy Journal, 112(4), 2916 - 2927. Palmer, G. K. & Pearce, R. C. (1999). Production Practices, Light Air-cured Tobacco. In: Tobacco Production, Chemistry and Technology. Editors: Davis, D. and Nielsen, M., Blackwell Science, Cambridge, 143 - 153. Peedin, G. F. (1999). Production Practices, Flue-cured Tobacco. In: Tobacco Production, Chemistry and Technology. Editors: Davis, D. and Nielsen, M., Blackwell Science, Cambridge, 104 - 142. Peele, T. C., Webb, H. J. & Bullock, J. F. (1960). Chemical Composition of Irrigation Waters in the South Carolina Coastal Plain and Effects of Chlorides in Irrigation Water on the Quality of Flue-Cured Tobacco. Agronomy Journal, 52(8), 464 - 467. Rosales, M. A., Franco-Navarro, J. D., Peinado-Torrubia, P., Díaz-Rueda, P., Álvarez, R. & Colmenero-Flores, J. M. (2020). Chloride Improves Nitrate Utilization and NUE in Plants. Frontiers in Plant Science, 11, 442. Shang, J., Xu, J. Y., Fan, Y. K., Huang, W. X. & Xu, Z.C. (2017). Physiological responses of flue-cured tobacco under the high chloride to chloride channel inhibitors. Journal of Plant Nutrition and Fertilizers, 23(2), 460 - 467. Tabaxi, I., Roussis, I., Karydogianni, S., Kakabouki, I., Kalivas, A., Folina, A. E. & Bilalis, D. (2020). Effect of chloride content in irrigation water on yield and chemical composition of leaves of three oriental tobacco (Nicotiana tabacum L.) cultivars. Journal of Elementology, 25(2), 581 - 594. Tan, J., Liu, X., Li, Q., Zhou, J., Chen, L., Bin, J., Wang, Q. & Li, P. (2016). Study on Characteristics and Influencing Factors of Chloride of Soils and Tobacco Leaves in Wenshan Tobacco Growing Areas. Chinese tobacco science, 37(5), 40 - 46 (Chinese). Tang, Z., Chen, L., Chen, Z., Fu, Y., Sun, X., Wang, B. & Xia, T. (2020). Climatic factors determine the yield and quality of Honghe flue-cured tobacco. Scientific Reports, 10, 19868. Tiecher, T., Pace, C. R., Gatiboni, L., Vann, M., Hardy, D. & Fisher, L. (2023). Flue-cured tobacco and Cl rates: Implications on yield, quality, and nutrient concentration. Agronomy Journal, 115(2), 896 - 908. Tso, T. C. (1999). Seed to Smoke. In: Tobacco Production, Chemistry and Technology. Editors: Davis, D. and Nielsen, M., Blackwell Science, Cambridge, 1 - 31. Wang, L., Xu, J. Y., Jia, W., Chen, Z. & Xu, Z. C. (2020). Chloride salinity in a chloride-sensitive plant: Focusing on photosynthesis, hormone synthesis and transduction in tobacco. Plant Physiology and Biochemistry, 153, 119 - 130. Wang, Y., Zhou, H., Xu, L. & Ye, X. (2023). Salt tolerance of two different varieties of tobacco under NaCl stress. Research Square, 1(1), 1 - 27. White, P. J. & Broadley, M. R. (2001). Chloride in Soils and Its Uptake and Movement within the Plant: A Review. Annals of Botany, 88(6), 967 - 988. Wu, D., Hu, Y., Liu, Y. & Zhang, R. (2021). Review of Chloride Ion Detection Technology in Water. Applied Sciences, 11, 11137. Xiao, Y., Liao, Y., Hou, J., Li, L., Xu, T., Ma, F., Yu, F., Tan, Z., He, Z., Jian, H., Li, H. & He, B. (2022). Evolution trend of soil fertility in tobacco-planting area of Chenzhou, Hunan Province, China. Open Life Sciences, 17(1), 1568 - 1578. Xu, G., Magen, H., Tarchitzky, J. & Kafkafi, U. (1999). Advances in Chloride Nutrition of Plants. Advances in Agronomy, 68, 97 - 150. Yamini, S., Ravindra Babu, P., Prasad, P. R. K. & Martin Luther, M. (2018). Secondary nutrients and chloride content of tobacco growing soils of Prakasam district, Andhra Pradesh. Journal of Pharmacognosy and Phytochemistry, 7(6), 134 - 137. |
|
| Date published: 2026-02-25
Download full text