Biochemical blood parameters in Balkan donkeys (Equus asinus) in Bulgaria
Аnton Rusenov

, Nikolina Rusenova

Abstract: The Balkan donkey is an autochthonous breed, traditionally raised by livestock breeders on the Balkan Peninsula. Its population in Bulgaria is decreasing, ageing, and even threatened with extinction. Therefore, the awareness of the physiological and pathological conditions of the Balkan donkey is essential for the conservation of the breed.
The present study aimed to investigate a large number of blood biochemical parameters in healthy Balkan donkeys in Bulgaria and to determine their gender- and age-related changes. Blood sera from 73 clinically healthy Balkan donkeys were collected to assay 20 biochemical blood parameters using a Mindray BS-120 biochemical analyzer and an Electrolyte Analyzer/Caretium: XI–921A. Statistical analysis of the gender and age effects on the measured variables was performed with the non-parametric Kruskal-Wallis test.
Regarding gender, significant differences were observed in the following biochemical parameters: Glu, CREA, TG, TP, ASAT, ALAT, ALP, Ca, and Cl. Additionally, significant differences were noted in age for LDH.
The study emphasizes the need to consider animal gender and age when interpreting blood biochemical parameters about the protection of animal genetic resources.
Keywords: Balkan donkeys; biochemical parameters; blood; Bulgaria; healthy
Citation: Rusenov, A. & Rusenova, N. (2025). Biochemical blood parameters in Balkan donkeys (Equus asinus) in Bulgaria. Bulg. J. Agric. Sci., 31(5), 1046–1052
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| Date published: 2025-10-28
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