Breeding qualities of monogerm dihaploid sugar beet lines
Georgi Kikindonov
Abstract: The successful induction of sugar beet haploids through in vitro cultivation of unpollinated ovules, followed by chromosome doubling of the regenerants, enables the discovery and stabilization of valuable gene recombinants. The objects of our study are doubled regenerants, obtained by in vitro unpollinated ovules of individually selected hybrid monogerm plants. The pollen fertility of the monogerm dihaploids is high; they exhibit full gametophytic self-compatibility, which allows for their use as “O” types of monogerm MS lines. The higher indices of productivity and technological qualities of the dihaploid lines, respectively, their MS analogues, are a precondition for their better appearance as parental components in hybridization. The combining ability of the tested monogerm dihaploid lines regarding the root yield in their triploid crosses is exceptionally high. The 15-4 line has proven higher productivity in its triploid crosses, and the diploid hybrids of 55-4 yield high amounts of root and white sugar per unit area.
Keywords: dihaploids; hybrids; monogerm; sugar beet; white sugar yield
Citation: Kikindonov, G. (2025). Breeding qualities of monogerm dihaploid sugar beet lines. Bulg. J. Agric. Sci., 31(5), 991–996
| References: (click to open/close) | Abramova, Z.V. (1985). Genetics of Programmed Teaching, Agropromizdat, Moscow. 259 - 265 (Ru).
Antonov, I. (1982). Breeding of monogerm sugar beet varieties. Thesis. Shumen, 233 - 236 (Bg).
Antonov, I. (1997). Basic trends in the breeding of monogerm male sterile lines of sugar beet. Plant Science, 34(7 - 8), 24 - 27 (Bg).
Atanassov, A. I. (1988). Biotechnology in plant growing. Zemizdat, Sofia, 212 (Bg).
Bosemark, N. O. (2006). Genetics and breeding. In: Draycott, A.P., editor. Sugar beet. Oxford: Blackwell; 2006, 50 - 88.
Bossoutrot, D. & Hosemans, D. (1985). Gynogenesis in Beta vulgaris L.: From in vitro culture of unpollinated ovules to the production of doubled haploid plants in soil. Plant Cell Rep., 4, 300 - 303.
Dirim, E., Say, A. & Arslan, M. (2023). Assessment of genetic diversity, yield and quality performance of O-type Sugar Beet (Beta vulgaris L.) breeding lines. Genetic Resources and Crop Evolution, 71(4), 1605 - 1614.
Gurel, E., Gurel, S. & Lemaux, P. G. (2008). Biotechnology applications for Sugar Beet. Critical Reviews in Plant Sciences, 27(2), 108 - 140.
Gurel, S., Pazuki, A., Aflaki, F. & Gurel, E. (2021). Production of doubled haploid sugar beet (Beta vulgaris L.) plants through gynogenesis. Methods Mol.Biol., 2289, 313 - 323.
Hansen, A. L., Plever, C., Pedersen, H. C., Keimer, B. & Andersen, S.B. (1994). Efficient in vitro chromosome doubling during Beta Vulgaris ovule culture. Plant Breeding, 112(2), 89 - 95.
Kolesnikova, O., Donskikh, E. & Berdnikov, R. (2021). Haploid technology as a tool for creating a selection material for Sugar beets. Vavilov Journal of Genetics and Breeding, 25(8), 812-821. (Ru)
Lidanski, T. (1988). Statistical methods in biology and agriculture. Zemizdat, Sofia, 102 - 104 (Bg).
Lux, H. & Wetzel, C. (1990). Production of haploid sugar beet (Beta vulgaris L.) by culturing unpollinated ovules. Plant Breeding, 104(3), 177 - 183.
Mather, K. & Jinks, J. L. (1996). Biometrical Genetics.3rd edn. Chapman and Hall. London.
Mezei, S., Kovachev, L., Chachich, N. & Nagl, N. (2002). In vitro propagation and development of dihaploids in sugarbeet breeding. Biotechnology and Biotechnological Equipment, 16(2), 58 - 61.
Nichols, J. B., Dalton, C. C., Todd, G. A. & Broughton, N. W. (1992). Genetic engineering of sugar beet. Zuckerindustrie , 117(10), 797 - 800.
Pausheva, Z. (1988). Plant cytology practicum.108 - 211 (Ru).
Ragot, M. & Steen, P. (1992). Genetic and environmental effects on chromosome doubling of sugar beet (Beta vulgaris L.) haploids. Euphytica, 63(3), 233 - 237.
Seeja, G. & Sreekumars, S. (2020). Doubled haploids in genetic improvement: A review. Int. Jour. of Recent Sci. Research, 11(01), (D), 36941 - 36949.
Slavova, J. (1993). Effective method for sugar beet haploids obtained from unpollinated ovules. Journal of Biotechnology and Biotechnological Equipment, 7(2), 34 - 35.
Steen, P. (1987). The role of biotechnology in breeding improved sugar beet varieties. Brit.Sugar Beet Rev., 55(4), 54 - 55.
Svirshchevskaya, A. M. & Dolezel, J. (2000). Production and performance of gynogenetic sugarbeet lines. J.Sugar Beet Res., 37(4), 117 - 133.
Van Geyt, J., Speckmann, G. J.jr, D’Halluin, K. & Jacobs, M. (1987). In vitro induction of haploid plants from unpollinated ovules and ovaries of sugar beet (Beta vulgaris L.), Theor.Appl.Genet., 73(6), 920 - 925.
Vasilchenko, E. N., Zemlyanukhina, O. A., Karpechenko, N. & Kalaev, V. (2021). Technology of sugar beet dihaploid lines development. Acta Horticulturae, 1324, 131 - 136.
Zakhariev, A. (1973). Possibilities of obtaining high percentage of tetraploid C1 plants of sugar beet. Genetics and Breeding, 6(6), 497 - 499 (Bg).
Zakhariev, A. & Kikindonov, G. (1997). Possibilities of haploidy application in sugar beet breeding, Plant Science, 34(7-8), 28 - 30 (Bg).
Zhuzhalova, T., Kolesnikova, E., Vasilchenko, E. & Cherkasova, N. (2020). Biotechnological methods as a tool for efficient sugarbeet breeding. Vavilovski Zhurnal Genetiki i Selektsii, 24(1), 40 - 47 (Ru). |
|
| Date published: 2025-10-28
Download full text 
