AMMI and GGE biplot analyses of adaptability and yield stability of advantage varieties of common winter wheat
Gergana Nikolova Desheva

, Manol Georgiev Deshev

, Evgeniya K. Valchinova

, Bozhidar Nikolov Kyosev

Abstract: The creation of varieties that can be adapted to a wide range of environments is the crucial goal of plant breeders in wheat improvement programs. The primary objective of this study was to identify high-yielding, stable, and adaptive common winter wheat genotypes for breeding purposes by applying various statistical analyses. The study was conducted over four growing seasons in the experimental field of IPGR-Sadovo, Southern Bulgaria. In the study, 46 common winter wheat cultivars with different geographical origins were included. The experiments were arranged in a completely randomized block design with three replications on plots of 10 m². Combined analysis of variance (ANOVA) revealed that grain yield was significantly affected by the sources of variance: genotype (G), environment (E), and the genotype × environment interaction (GEI) (p < 0.001). Genotypes G16, G31, G14, G24, and G1 emerged as the most stable according to the parametric stability estimates analysis. Genotypes G14, G33, and G19 were considered generally adaptable to each environment according to AMMI1 analysis, while G19, G2, G35, G21, G4, and G32 were assessed as the most stable according to AMMI2 analysis. GGE biplot analyses identified G33, G14, G16, and G9 as the most favorable and adaptable to a wide range of environments that could be included in the standard winter wheat breeding programs.
Keywords: AMMI analysis; GGE analysis; stability parameters; wheat; yield
Citation: Desheva, G. N., Deshev, M. G., Valchinova, E. K. & Kyosev, B. N. (2025). AMMI and GGE biplot analyses of adaptability and yield stability of advantage varieties of common winter wheat. Bulg. J. Agric. Sci., 31(5), 940–950
| References: (click to open/close) | Adil, N., Wani, S. H., Rafiqee, S., Mehrajuddin, S. O. F. I., Sofi, N. R., Shikari, A. B., Hussain, A., Mohiddin, F., Jehangir, I. A., Khan, G. H., Sofi, M. A., Sheikh, F. A., Bhat, M. A., Khan, M. N., Dar, Z. A. & Rahimi, M. (2022). Deciphering genotype × environment interaction by AMMI and GGE biplot analysis among elite wheat (Triticum aestivum L.) genotypes of Himalayan region. Ekin Journal of Crop Breeding and Genetic, 8(1), 41 - 52. Ahakpaz, F., Majidi, H. E., Roostaei, M., Bihamta, M. R. & Mohammadi, S. (2023). Comprehensive stability analysis of wheat genotypes through multi-environmental trials. Journal of Agricultural Sciences (Tarim Bilimleri Dergisi), 29(1), 317 - 334. Akcura, M. & Kaya, Y. (2008). Nonparametric stability methods for interpreting genotype by environment interaction of bread wheat genotypes (Triticum aestivum L.). Genetics and Molecular Biology, 31(4), 906 - 913. Ali, M. B., El-Sadek, A. N., Sayed, M. A. & Hassaan, M. A. (2015). AMMI biplot analysis of genotype × environment interaction in wheat in Egypt. Egyptian Journal of Plant Breeding, 19(6), 1889 - 1901. Altay, F. (2012). Yield stability of some Turkish winter wheat (Triticum aestivum L.) genotypes in the western transitional zone of Turkey. Turkish Journal of Field Crops, 17(2), 129 - 134. Amare, A., Mekbib, F., Tadesse, W. & Tesfaye, K. (2020). Genotype x environment interaction and stability of drought tolerant bread wheat (Triticum aestivum L.) genotypes in Ethiopia. International Journal of Research Studies in Agricultural Sciences, 6(3), 26 - 35. Arain, M. A., Sial, M. A., Rajput, M. A. & Mirbahar, A. A. (2011). Yield stability in bread wheat genotypes. Pakistan Journal of Botany, 43(4), 2071 - 2074. Awoke, S. & Sharma, M. K. (2016). Parametric and non-parametric methods to describe genotype by environment interaction and grain yield stability of bread wheat. Statistics and Applications, 14(1&2), 9 - 29. Baktash, K. (2016). AMMI Analysis of adaptability and yield stability of promosing lines of bread wheat (Triticum aestavum L.). The Iraqi Journal of Agricultural Sciences, 47(Special Issue), 35 - 43. Bishwas, K. C., Poudel, M. R. & Regmi, D. (2021). AMMI and GGE biplot analysis of yield of different elite wheat line under terminal heat stress and irrigated environments. Heliyon, 7(6), e07206. Cheshkova, A. F., Stepochkin, P. I., Aleynikov, A. F., Grebennikova, I. G. & Ponomarenko, V. I. (2020). A comparison of statistical methods for assessing winter wheat grain yield stability. Vavilovskyi Journal Genetiki i Selekcii, 24(3), 267 - 275. Chimdesa, O., Asefa, K., Alemu, S. & Teshome, G. (2019). Evaluating agronomic performance and yield stability of improved bread wheat varieties across low moisture stress areas of Guji Zone, Southern Oromia. International Journal of Research in Agriculture and Forestry, 6(5), 1 - 10. Dabi, A., Alemu, G., Sime, B., Geleta, N., Delessa, A., Solomon, T., Zegaye, H., Asnake, D., Asefa, B., Duga, R., Shewaye, Y., Getamesay, A., Zewudu, D., Girma, B., Badebo, A. & Abeyo, B. (2023) Genotype×environment interaction and stability analysis using GGE biplot for grain yield of bread wheat (Triticum aestivum L.) genotypes under low moisture stress areas of Ethiopia. International Journal of Bio-resource and Stress Management, 14(7), 1089 - 1098. Eberhart, S. A. & Russell, W. A. (1966). Stability parameters for comparing varieties 1. Crop Science, 6(1), 36 - 40. Farshadfar, E. (2008). Incorporation of AMMI stability value and grain yield in a single non-parametric index (GSI) in bread wheat. Pakistan Journal of Biological Science, 11(14), 1791 – 1796. Farshadfar, E., Mahmodi, N. & Yaghotipoor, A. (2011). AMMI stability value and simultaneous estimation of yield and yield stability in bread wheat (Triticum aestivum L.). Australian Journal of Crop Science, 5(13), 1837 - 1844. Finlay, K. W. & Wilkinson, G. N. (1963). The analysis of adaptation in a plant breeding programme. Australian Journal of Agricultural Research, 14(6), 742 - 754. Francis, T. R. & Kannenberg, L. W. (1978). Yield stability studies in short-season maize: I. A descriptive method for grouping genotypes. Canadian Journal of Plant Science, 58(4), 1029 - 1034. Gupta, V., Kumar, M., Singh, V., Chaudhary, L., Yashveer, S., Sheoran, R., Dalal, M.S., Nain, A., Lamba, K., Gangadharaiah, N., Sharma, R. & Nagpal, Sh. (2022). Genotype by environment interaction analysis for grain yield of wheat (Triticum aestivum (L.) em.Thell) genotypes. Agriculture, 12(7), 1002. Gupta, V., Mehta, G., Kumar, S., Ramadas, S., Tiwari, R., Singh, G. P. & Sharma, P. (2023). AMMI and GGE biplot analysis of yield under terminal heat tolerance in wheat. Molecular Biology Reports, 50(4), 3459 - 3467. Hayder, F. M. A., Uddin, M. N., Roy, U. K., Uddin, G. M. & Siddique, A. B. (2018). Stability analysis of yield performance in wheat (Triticum aestivum L.). Plant, Environment Development, 7(2), 10 - 15. Huhn, M. (1990). Nonparametric measures of phenotypic stability. Part 1: Theory. Euphytica 47(3), 189–194. Jędzura, S., Bocianowski, J. & Matysik, P. (2023). The AMMI model application to analyze the genotype–environmental interaction of spring wheat grain yield for the breeding program purposes. Cereal Research Communications, 51(1), 197 - 205. Kamini, D., Sharma, D. J., Agrawal, A. P. & Pandey, D. (2020). Stability analysis in wheat (Triticum aestivum L.). Journal of Pharmacognosy and Phytochemistry, 9(5S), 295 - 298. Kang, M. S. (1988). A rank-sum method for selecting high-yielding, stable corn genotypes. Cereal Research Communication, 16(1/2), 113 - 115. Kebede, G., Worku, W., Feyissa, F. & Jifar, H. (2023). Genotype by environment interaction and stability analysis for selection of superior fodder yield performing oat (Avena sativa L.) genotypes using GGE biplot in Ethiopia. Ecological Genetics and Genomics, 28, 100192. Khan, M. A. U., Mohammad, F., Khan, F. U., Ahmad, S., Raza, M. A. & Kamal, T. (2020). Comparison among different stability models for yield in bread wheat. Sarhad Journal of Agriculture, 36(1), 282 - 290. Knapp, S., Brabant, C., Oberforster, M., Grausgruber, H. & Hiltbrunner, J. (2017). Quality traits in winter wheat: Comparison of stability parameters and correlations between traits regarding their stability. Journal of Cereal Science, 77, 186 - 193. Krupal, S. M., Rathod, S. T. & Kamble, B. G. (2018). Stability analysis for yield and quality traits in wheat (Triticum aestivum L.). Electronic Journal of Plant Breeding, 9(1), 160 - 168. Kyratzis, A. C., Pallides, A. & Katsiotis, A. (2022). Investigating stability parameters for agronomic and quality traits of durum wheat grown under Mediterranean conditions. Agronomy, 12(8), 1774. Mansour, E., Moustafa, E. S. A., El-Naggar, N. Z. A., Abdelsalam, A. & Igartua, E. (2018). Grain yield stability of high-yielding barley genotypes under Egyptian conditions for enhancing resilience to climate change. Crop and Pasture Science, 69(7), 681 - 690. Mehari, M., Tesfay, M., Yirga, H., Mesele, A., Abebe, T., Workineh, A. & Amare, B. (2015). GGE biplot analysis of genotype-by-environment interaction and grain yield stability of bread wheat genotypes in South Tigray, Ethiopia. Communications in Biometry and Crop Science, 10(1), 17 - 26. Melkamu, M. T., Sentayehu, S. A. & Eticha, F. (2015). GGE biplot analysis of genotype by environment interaction and grain yield stability of bread wheat genotypes in South East Ethiopia. World Journal of Agricultural Sciences, 11(4), 183 - 190. Mohammadi, M., Ghojigh, H., Khanzadeh, H., Hosseinpour, T. & Armion, M. (2016). Assessment of yield stability of spring bread wheat genotypes in multi environment trials under rainfed conditions of Iran using the AMMI model. Crop Breeding Journal, 6(2), 59 - 66. Mohammadi, R., Jafarzadeh, J., Poursiahbidi, M. M., Hatamzadeh, H. & Amri, A. (2023). Genotype-by-environment interaction and stability analysis for grain yield in durum wheat using GGE biplot and genotypic and environmental covariates. Agricultural Research, 12(4), 364 - 374. Munjal, R., Ram, K., Kesh, H., Suresh, N. & Kumari, A. (2020). AMMI and GGE biplot analysis for yield stability of wheat genotypes under drought and high temperature stress. International Journal of Current Microbiology and Applied Sciences, 9(5), 377 - 389. Nassar, R. & Huhn, M. (1987). Studies on estimation of phenotypic stability: tests of significance for nonparametric measures of phenotypic stability. Biometrics, 43(1), 45 - 53. Neisse, A. C., Kirch, J. L. & Hongyu, K. (2018). AMMI and GGE Biplot for genotype × environment interaction: a medoid–based hierarchical cluster analysis approach for high–dimensional data. Biometrical Letter, 55(2), 97 - 121. Obsa, C. (2019). Evaluating agronomic performance and yield stability of improved bread wheat varieties across low moisture stress areas of Guji Zone, Southern Oromia. Agricultural Research and Technology: Open Access Journal, 22(1), 556183. Omrani, A., Omrani, S., Khodarahmi, M., Shojaei, S. H., Illés, Á., Bojtor, C., Mousavi, S. M. N. & Nagy, J. (2022). Evaluation of grain yield stability in some selected wheat genotypes using AMMI and GGE biplot methods. Agronomy, 12(5), 1130. Pinthus, M. J. (1973). Estimate of genotypic value: A proposed method. Euphytica, 22(1), 121 - 123. Plaisted, R. I. & Peterson, L. C. (1959). A technique for evaluating the ability of selection to yield consistently in different locations or seasons. American Potato Journal, 36, 381 - 385. Plaisted, R. L. (1960). A shorter method for evaluating the ability of selections to yield consistently over locations. American Potato Journal, 37, 166 - 172. Poudel, M. R., Ghimire, S., Pandey, M. P., Dhakal, K., Thapa, D. B. & Poudel, H. K. (2020). Yield stability analysis of wheat genotypes at irrigated, heat stress and drought condition. Journal of Biology and Today's World, 9(5), 220. Pour-Aboughadareh, A., Yousefian, M., Moradkhani, H., Poczai, P. & Siddique, K. H. M. (2019). Stabilitysoft: A new online program to calculate parametric and non-parametric stability statistics for crop traits. Applied Plant Science, 7(1), e01211. Pour-Aboughadareh, A., Khalili, M., Poczai, P. & Olivoto, T. (2022). Stability indices to deciphering the genotype-by-environment interaction (GEI) effect: An applicable review for use in plant breeding programs. Plants, 11(3), 414. Purchase, J. L. (1997). Parametric analysis to describe genotype by environment interaction and yield stability in winter wheat. Ph.D. Thesis, Department of Agronomy, Faculty of Agriculture of the University of the Free State, Bloemfontein, South Africa. Purchase, J. L., Hatting, H. & van Deventer, C. S. (2000). Genotype × environment interaction of winter wheat (Triticum aestivum L.) in South Africa: II. Stability analysis of yield performance. South African Journal of Plant and Soil, 17(3), 101 - 107. Ram, K., Munja, R., Kesh, H.,Suresh & Kumari, A. (2020). AMMI and GGE biplot analysis for yield stability of wheat genotypes under drought and high temperature stress. International Journal of Current Microbiology and Applied Sciences, 9(5): 377-389 9. Regmi, D., Poudel, M. R., Bishwas, K. C. & Poudel, P. B. (2021). Yield stability of different elite wheat lines under drought and irrigated environments using AMMI and GGE biplots. International Journal of Applied Science and Biotechnology, 9(2), 98 - 106. Roostaei, M., Mohammadi, R. & Amri, A. (2014). Rank correlation among different statistical models in ranking of winter wheat genotypes. The Crop Journal, 2(2-3), 154 - 163. Shukla, G. K. (1972). Some statistical aspects of partitioning genotype-environmental components of variability. Heredity, 29(2), 237 - 245. Sial, M. A., Dahot, M. U., Mangrio, S. M., Mangan, B. N., Arain, M. A., Naqvi, M. H. & Memon, Sh. (2007). Genotype x environment interaction for grain yield of wheat genotypes tested under water stress conditions. Science International, 19(2), 133 - 137. Singh, C., Srivastava, P., Sharma, A., Kumar, P., Chhuneja, P., Sohu, V. S. & Bains, N. S. (2018). Stability analysis for grain yield and some quality traits in bread wheat (Triticum aestivum L.). Journal of Applied and Natural Science, 10(1), 466 - 474. Singh, C., Gupta, A., Gupta, V., Kumar, P., Sendhil, R., Tyagi, B. S., Singh, G., Chatrath, R. & Singh, G. P. (2019). Genotype x environment interaction analysis of multi-environment wheat trials in India using AMMI and GGE biplot models. Crop Breeding and Applied Biotechnology, 19, 309 - 318. Subedi, M., Cárcamo, H. A., Knodel, J. J., Weaver, D. K., Cuthbert, R. D., Pozniak, C. J., Nilsen, K. T. & Beres, B. L. (2021). Stability analysis of stem solidness, grain yield, and grain protein concentration in spring wheat. Canadian Journal of Plant Science, 101(4), 456 - 475. Tanin, M. J., Sharma, A., Saini, D. K., Singh, S., Kashyap, L., Srivastava, P., Mavi, G. S., Kaur, S., Kumar, V., Kumar, V., Grover, G., Chhuneja, P. & Sohu, V. S. (2022). Ascertaining yield and grain protein content stability in wheat genotypes having the Gpc-B1 gene using univariate, multivariate, and correlation analysis. Frontiers in Genetics, 13, 1001904. Temesgen, M., Alamerew, S., Eticha, F. & Mehari, M. (2015). Genotype X environment interaction and yield stability of bread wheat genotypes in South East Ethiopia. World Journal of Agricultural Sciences, 11(3), 121 - 127. Thakur, P., Prasad, L. C., Prasad, R., Omprakh, Chandra, K. & Rashmi, K. (2019). Stability analysis for yield and related traits over four environments in wheat (Triticum aestivum L.). Plant Archives, 19(2), 3541 - 3545. Tollenaar, M. & Lee, E. A. (2002). Yield potential, yield stability and stress tolerance in maize. Field Crops Research, 75(2-3), 161 - 169. Tulu, L. & Wondimu, A. (2019). Adaptability and yield stability of bread wheat (Triticum aestivum) varieties studied using GGE-biplot analysis in the highland environments of South-western Ethiopia. African Journal of Plant Science, 13(6), 153 - 162. van Eeuwijk, F. A., Bustos-Korts, D. V. & Malosetti, M. (2016). What should students in plant breeding know about the statistical aspects of genotype × environment interactions? Crop Science, 56(5), 2119 - 2140. Wardofa, G. A., Asnake, D. & Mohammed, H. (2019). GGE biplot analysis of genotype by environment interaction and grain yield stability of bread wheat genotypes in Central Ethiopia. Journal of Plant Breeding and Genetic, 7(2), 75 - 85. Wardofa, G. A. & Ararsa, A. D. (2020). Evaluation of grain yield stability analysis in bread wheat (Triticum aestivum L.) genotypes using parametric method. American Journal of Life Sciences, 8(6), 189 - 195. Wricke, G. (1962). About a method for assessing ecological variance in field experiments. Journal of Plant Breeding, 47, 92 – 96 (De). Yang, R. C., Crossa, J., Cornelius, P. L. & Burgueno, J. (2009). Biplot analysis of genotype x environment interaction: proceed with caution. Crop Science, 49(5), 1564 - 1576. |
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| Date published: 2025-10-28
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