Molecular identification and characterization of isolated bacteria from beef meat by 16s rRNA gene analysis
Noor Muthanna Mahmood
, Mohammed Munis Dakheel
Abstract: The present study is focused on the molecular identification of 16s rRNA genes, identifying bacterial strains from local beef shops, and analyzing the patterns of PCR products collected from various stores in Baghdad, Iraq. For the purpose of this study, twenty beef steak samples were randomly chosen from nearby marketplaces. The total number of bacteria was counted to identify various bacterial strains presented in the samples. PCR methods were used to detect 16S rRNA extraction patterns and DNA gene primer preparation patterns, and the results revealed bacterial contamination of meat, specifically beef, sold in local butcher shops. Moreover, these 16S ribosomal RNA genes were identified via the corresponding Genbank accession codes and through molecular identification of many bacterial species, including Staphylococcus warneri, Serratia marcescens, Klebsiella oxytoca, and Raoultella ornithinolytica; however, the most dominant bacterial species were Serratia marcescens, which was identified utilizing 16S rRNA and DNA sequences examined utilizing nucleotide BLAST alignment techniques. In brief, the findings of this study indicated that beef exposure to various environmental factors in Boucher shops significantly influenced beef growth, especially that of several bacterial species; further research is thus required to identify additional specific genes for additional bacterial types.
Keywords: 16S rRNA; Beef meat; DNA genes; molecular detection; total bacterial count
Citation: Mahmood, N. M. & Dakheel, M. M. (2025). Molecular identification and characterization of isolated bacteria from beef meat by 16s rRNA gene analysis. Bulg. J. Agric. Sci., 31(4), 800–806.
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| Date published: 2025-08-27
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