Genotyping of Progesterone receptor gene in two rabbit breeds using PCR-RFLP assay
Milena Bozhilova-Sakova
, Denis Viryanski
, Ivona Dimitrova
, Maya Ignatova
Abstract: The purpose of the present experimental work was to define the genetic status of the rabbit progesterone receptor gene (PGR), associated with reproduction traits and litter size in rabbits. For that matter were used 80 does of New Zealand White (NZW) and Californian (CA) rabbit breeds. Blood samples were collected in vacuum tubes and then stored at -20°C until DNA purification. The genetic polymorphism was determined by the PCR-RFLP method using the specific set of primers and the restriction enzyme Eco31I. In both breeds were identified all three possible genotypes (GG, AG and AA) of 2464G > A SNP for PGR gene, and in both studied breeds the frequency of homozygous genotype GG was higher than the other genotypes – 0.73 for NZW and 0.55 for CA. The genotype AA was with lowest frequency in both breeds – 0.12 for NZW and 0.07 for CA. The results in NZW breed were not consisted with HWE and there was observed statistically significant difference with p=0.005. This is the first study in Bulgaria involving genetic diversity of PGR in rabbits. Further experiments are pending to associate the influence of each genotype with the litter size and milk production in rabbits.
Keywords: PCR-RFLP; polymorphism; progesterone receptor gene; rabbit breeding
Citation: Bozhilova-Sakova, M., Viryanski, D., Dimitrova, I. & Ignatova, M. (2024). Genotyping of Progesterone receptor gene in two rabbit breeds using PCR-RFLP assay. Bulg. J. Agric. Sci., 30(6), 1102–1105
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| Date published: 2024-12-16
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