Evaluation of grain yield and quality traits and identification of seed storage protein alleles in durum wheat genotypes
Sonya Donevа, Krasimira Tanevа, Rangel Dragov, Violeta Bozhanova
Abstract: The aim of modern selection is the creation of high-yielding varieties with improved grain quality. The investigation included collection of 8 durum wheat genotypes: Mirela, Heliks, Saya, MVPennedur, Мarco Aurelio, Wintergold, D-8243, DF-009114002 and Agridur. Cultivars Heliks and Saya and line D-8243, were the highest yielding among all studied genotypes; averaged for three years, their yield varied from 530.8 kg/da (breeding line D-8243) to 644.1 kg/da (cultivar Heliks). According to the trait gluten strength, the highest yielding genotypes had the lowest SDS-sedimentation volume, which varied from 19.7 сm3 in cultivar Heliks to 21 сm3 in line D-8243, and respectively had the lowest SDS values. All other investigated genotypes were characterized by statistically significant higher values of the SDS-sedimentation volume, the variation being from 42.8 сm3 in cultivar Wintergold to 73.5 сm3 in line DF-009114002. Using the method of electrophoresis, the allele composition of the γ-gliadins, the high molecular (HMW-GS) and low molecular (LMW-GS) glutenins was studied. Six alleles of HMW were identified, which were coded for by loci Glu-A1 and Glu-B1. In the LMW zone, eight alleles were expressed (loci Glu-A3 and Glu-B3). The accessions having gliadin subunits γ-42 (Heliks, Saya and D-8243) and γ-45 (Mirela, MVPennedur, Marco Aurelio, Wintergold and DF-009114002) in locus Gli-B1 were determined. Depending on the respective allele forms in loci GliB1/GluB3, the investigated genotypes were divided into two main types (LMW-1 and LMW-2), which determined the quality of the ground semolina. The quality scores and the genetic variability were calculated for the separate loci. Based on the obtained results, varieties Heliks, Saya by high-yield and Marco Aurelio, Mirela by high quality were selected as parental components for crosses.
Keywords: grain yield; HMW-GS; LMW-GS; SDS-PAGE; Triticum durum; γ-gliadin; А-PAGE
Citation: Doneva, S., Taneva, K., Dragov. R. & Bozhanova, V. (2023). Evaluation of grain yield and quality traits and identification of seed storage protein alleles in durum wheat genotypes. Bulg. J. Agric. Sci., 29(6), 1170–1180.
References: (click to open/close) | Aguiriano, E., Ruiz, M., Fité, R. & Carrillo, J. (2008). Genetic variation for glutenin and gliadins associated with quality in durum wheat (Triticum turgidum L.ssp. turgidum) landraces from Spain. Spanish Journal of Agricultural Research, 6(4), 599-609. Babay, E., Hanana, M., Mzid, R., Slim-Amara, H., Carrillo, J. & Rodriguez-Quijano, M. (2015). Influence of allelic prolamin variation and localities on durum wheat quality. Journal of Cereal Science, 63, 27-34. Bahraei, S., Saidi, A. & Alizadeh, D. (2004). High molecular weight glutenin subunits of current bread wheat grown in Iran. Euphytica, 137, 173-179. Boggini, G. & Pogna, N. (1989). The breadmaking quality and storage protein composition of Italian durum wheat. Journal of Cereal Science, 9(2), 131-138. Branlard, G. & Dardevet, M. (1985). Dversity of grain protein and bread wheat quality. II. Correlation between high molecular weight subunits of glutenin and flour characteristics. Journal of Cereal Science, 3, 345-354. Brites, C. & Carrillo, J. (2003). Inheritance of gliadin and glutenin protein in flour durum wheat crosses. Cereal Research Communications, 28, 239-246. Butow, B., Ma, W., Gale, K., Cornich, G., Rampling, L., Larroque, O., Morell, M. & Békés, F. (2003). Molecular discrimination of Bx7 alleles demonstrates that a higly expressed high-molecular-weight glutenin allele has a major impact on wheat flour dough strength. Theoretical and Applied Genetics, 107, 1524-1532. Chacón, E., Vázquez, F., Giraldo, P., Carrillo, J., Benavente, E. & Quijano, R. (2020). Allelic variation for prolamins in Spanish durum wheat landraces and its relationship with quality traits. Agronomy, 10(1), 136. Cherdouh, A., Khelifi, D., Carrillo, J. & Nieto-Taladriz, M. (2005). The high and low molecular weight glutenin subunit polymorphism of Algerian durum wheat landraces and old cultivars. Plant Breeding, 124(4), 338-342. Dechev, D., Bozhanova, V., Yanev, Sh., Delchev, G., Panayotova, G., Saldzhiev, I.,Nedyalkova, S., Hadzhiivanova, B. & Taneva, K. (2011). Achievements and problems of durum wheat breeding and technologies. Field Crop Studies, 7(1), 201-216. (BG) Doneva, S. (2017). Characterization of storage proteins of synthetic wheats in relation to their use as initial breeding material. Dissertation, General Toshevo, Bulgaria. (BG) Dragov, R. (2020). Combining ability for the grain wet gluten content in durum wheat. Bulgarian Journal of agricultural science, 26(5), 998-1002. Dragov, R. (2021). Gene action for grain protein content in durum wheat. Acta Agriculturae Slovenica, 117(2), 1-8. Duncan, D. (1955). Multiple Range and Multiple F-tests. Biometrics, II, 1-42. Fan, X., Song, Z., Kang, H., Yang, R. & Zhou, Y. (2009). Identification and characterization of HMWGSSubunits and their coding sequences in Dwarfing Polish Wheat. International Journal of Agricultural Research, 4(8), 237-249. Goutam, U., Kukreja, S., Tiwari, R., Chaudhury, A., Gupta, R., Dholakia, B. & Yadav, R. (2013). Biotechnological approaches for grain quality improvement in wheat: present status and future possibilities. Australian Journal of Crop Science, 7(4), 469-483. Gregová, E., Medvecká, E., Jómová, K. & Šliková, S. (2012). Characterization of durum wheat (Triticum durum Desf.) quality from gliadin and glutenin protein composition. Journal of Microbiology, Biotechnology and Food Sciences, 1, 610-615. Güleç, T., Yildirim, A., Koyuncu, M. & Sayaslan, A. (2019). Analysis of some Turkish durum wheat cultivars and landraces for MW-glutenin subunits. Journal of Aagricultural Faculty of Gaziosmanpasa, 36(1), 74-80. Gyurov, A. (1976). A study on the change of some quality indicators of durum wheat depending on weather conditions and on the question of its purchase. Bulgarian Journal of Crop Science, 8(3), 3-6 (Bg). Hamer, R., Weegels, P. & Marseille, J. (1992). Prediction of the Breadmaking quality of wheat: the use of HMW glutenin – a subunit-based quality scoring systems. Journal of Cereal Science, 15(1), 91-102. Hintum, T. & Elings, A. (1991). Assessment of glutenin and phenotypic diversity of Syrian durum wheat landraces in relation to their geographical origin. Euphytica, 55(3), 209-215. Igrejas, G., Guedes-Pinto, H., Carnide, V. & Branlard, G. (1999). The high and low molecular weight glutenin subunits and w-gliadin composition of bread and durum wheats commonly grown in Portugal. Plant Breeding, 118(4), 297-302. Ivanov, I. (1927). Durum wheat in Bulgaria (Triticum durum Desf.). Yearbook of Sofia University, 5, 97-178 (Bg). Кhan, K., McDonald, E. & Banasik, O. (1983). Polyacrylamide gel electrophoresis of gliadin proteins for wheat variety. Identification-procedural modifications and observations. Cereal Chemistry, 60(2), 178-181. Kilic, H., & Yagbasanlar, T. (2003). Studies on determination the genotype environment interaction of durum wheat (Triticum turgidum ssp. Durum) for some quality traits in the southeastern Anatolian conditions. 5th Field Crops Congress in Turkey, 13-17 October, Diyarbakir, Turkey, 180-185. Kudryavtsev, A., Illichevskii, N. Boggini, G. & Benedettelli, S. (1996). Gliadin polymorphism and genetic diversity of modern Italian durum wheat. Journal of Genetics and Breeding, 50, 239-248. Kudryavtsev, A., Melnikova, N. & Novoselskaya-Dragovich, A. (2014). Global durum wheat diversity: structure and origin revealed by means of the gliadin markers. Options Méditerranéennes: Série A. Séminaires Méditerranéens, 110, 57-61. Laemmli, U. (1970). Cleavage of structural proteins during the assembly of bacteriophage T4. Nature, 227, 680-685. Liu, C., Shepherd, K. & Rathjen, A. (1996). Improvement of durum wheat pastamaking and breadmaking qualities. Cereal Chemistry, 73(2), 155-166. Motalebi, M., Keshavarzi, M. & Naghavi, M. (2007). Glutenin subunit composition in durum (Triticum durum) landraces and cultivars. Asian Journal of Plant Sciences, 62(2), 399-402. Moragues, М., del Moral, L., Moralejo, M. & Royo, C. (2006). Yield formation strategies of durum wheat landraces with distinct pattern of dispersal within the Mediterranean basin I: Yield componentes. Field Crops Research, 95(2-3), 194-205. McIntosh, R., Dubcovsky, J., Rogers, J., Morris, C., Apples, R. & Xia, X. (2014). Catalogue of gene symbols for wheat: 2013-14 Supplement. Annual Wheat Newsletter, 58, 259-279. Nachit, M., Baum, M., Impiglia, A. & Ketata, H. (1995). Studies on some grain quality traits in durum wheat grown in Mediterranean environments. Options Méditerranéennes: Série A. Séminaires Méditerranéens, 22, 181-187. Nazco, R., Peña, R., Ammar, K., Villegas, D., Crossa, J., Moragues, M. & Royo, C. (2014). Variability in glutenin subunit composition of Mediterranean durum wheat germaplasm and its relationship with gluten editerr. Journal of Agricultural Science, 152(3), 379-393. Nei, M. (1973). Analisis of gene diversity in subdivided populations. Proceeding of the National Academy of Science of the USA, 70(12), 3321-3323. Nieto-Taladriz, M., Ruiz, M., Martinez, M., Vazquez, J. & Carrillo, J. (1997). Variation and classification of B-low –molecular-weight glutenin subunit alleles in durum wheat. Journal of Genetics and Breeding, 50, 239-248. Oak, M., Tamhankar, S., Rao, V. & Bhosale, S. (2004). Relationship of HMW, LMW Glutenin subunit and gliadin with gluten editerr in Indian Durum wheat. Journal of Plant Biochemistry and Biotechnology, 13, 51-55. Payne, P., Nightingale, M., Krattiger, A. & Holt, L. (1987). The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties. Journal of the Science of Food and Agriculture, 40(1), 51-65. Payne, P., Law, C. & Mudd, E. (1980). Control by homeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theoretical and Applied Genetics, 58, 113-120. Payne, P. & Lawrence, G. (1983). Catalogue of alleles for the editer gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for high-molecular weight subunit in hexaploid wheat. Cereal Research Communication, 11(1), 29-35. Payne, P., Jackson, E. & Holt, L. (1984). The association between γ-gliadin 45 and gluten editerr in durum wheat varieties: a direct casual effect or the result of genetic linkage? Journal of Cereal Science, 2(2), 73-81. Pogna, N., Autran, J., Mellini, F., Lafiandra, D., & Feiller, P. (1990). Chromosome 1B-encoded Gliadin and Glutenin subunits in Durum wheat: Genetics and relationship to gluten strength. Journal of Cereal Science, 11(1), 15-34. Porceddu, E., Turchetta, T., Masci, S., D’ovidio, R., Lafiandra, D., Kasarda, D., Impiglia, A. & Nachit, M. (1998). Variation in endosperm protein composition and technological quality properties in durum wheat. Euphytica, 100(1-3), 197-205. Raciti, C., Doust, M., Lombardo, G., Boggini, G. & Pecetti, L. (2003). Characterization of durum wheat editerranean germaplasm for high and low molecular weight glutenin subunits inrelation with quality. European Journal of Agronomy, 19(3), 373-382. Rapp, M., Lein, V., Lacoudre, F., Lafferty, J., Müller, E., Vida, G., Bozhanova, V., Ibraliu, A., Thorwarth, P., Piepho, H., Leiser, W., Würschum, T. & Longin, C. (2018). Simultaneous improvement of grain yield and protein content in durum wheat by different phenotypic indices and genomic selection. Theoretical and Applied Genetics, 131(6), 1315-1329. Ribeiro, M., Carvalho, C., Carnide, V., Guedes-Pinto, H. & Igrejas, G. (2011). Towards allelic diversity in the storage proteins of old and currently growing tetraploid and hexaploid wheats in Portugal. Genetic Resource and Crop Evolution, 58(7), 1051-1073. Roselló, M., Conxita, R., Álvaro, F., Villegas, D., Nazco, R. & Soriano, J. (2018). Pasta-Making quality QTLome from Mediterranean durum wheat landraces. Frontiers in Plant Science, 9, Article 1512. Ruiz, M., Bernal, G. & Giraldo, P. (2018). An update of low molecular weight glutenin subunits in durum wheat relevant to breeding for quality. Journal of Cereal Science, 83, 236-244. Sharma, A., Garg, S., Sheikh, I., Vyas, P. & Dhaliwal, H. (2020). Effect of wheat grain protein composition on end-use quality. Journal of Food Science and Technology, 57(11), 2771-2785. Singh, N. & Shepherd, K. (1988). Linkage mapping of genes controlling endosperm storage proteins in wheat. Genes on the short arms of group 1 chromosomes. Theoretical and Applied Genetics, 75, 628-641. Singh, N., Shepherd, K. & Cornish, G. (1991). A simplified SDS-PAGE procedure for separating LMW subunits of glutenin. Journal of Cereal Science, 14(3), 203-208. Taneva, К., Bozhanova, V. & Petrova, I. (2019). Variability, heritability and genetic advance of some grain quality traits and grain yield in durum wheat genotypes. Bulgarian Journal of Agricultural Science, 25(2), 288-295. Taneva, K., & Bozhanova, V. (2021). Use of marker assisted selection to improve the quality of durum wheat (Review). Bulgarian Journal of Crop Science, 58(6), 3-14 (Bg). Taneva, K. (2019). Study on a set of durum wheat genotypes and tetraploid related species according to quality-related properties of grain. Dissertation, Chirpan, Bulgaria (Bg). Todorov, I. (2006). Study on endosperm storage proteins and their use as genetic markers in wheat breeding. Dissertation, General Toshevo, Bulgaria (Bg). Todorovska, E., Zheleva, D., Christov, N., Jacquemin, J.-M., Fasoula, D., Ioannides, I., Bozhanova, V., Dechev, D., Atanassov, A. (2007). The molecular diversity of Bulgarian cereal germplasm collections – a reference point for better understanding, exploitation and broadening of the genetic base of cereal crops. 6th Plant Genomics European Meetings. Book of abstracts, 3-6 October, Tenerife, Spain.
|
|
| Date published: 2023-12-15
Download full text