GENETIC DIVERSITY IN PAPAVER BRACTEATUM AND PAPAVER SOMNIFERUM POPULATIONS REVEALED BY ISSR MARKERS

R. Darvishzadeh, I. Bernousi, B. A. Mandoulakani, Z. Mohseni

Abstract: Inter-simple sequence repeat (ISSR) markers were used to assess genetic diversity and structure of two populations of Papaver bracteatum and one population of Papaver somniferum. Fifteen ISSR primers amplifed 135 lociwhich 117 (86.6%) were polymorphic. The genetic diversity estimated by Shannon’s information index (I) was 0.283 and 0.1 for P. bracteatum and P. somniferum, respectively. Eighty-three (61%) fragments were found to be useful as species-specific markers and 52 fragments were conserved between two species. Analysis of molecular variance (AMOVA) showed that the genetic variation mostly allocated to among species (64%). UPGMA clustering algorithm differentiated and classified studied genotypes and populations in three distinct groups. The result of the present study indicated a wide range of variation in the phenetic relationship
among P. bracteatum and P. somniferum. Also, species-specifc bands generated could be used to develop the species-specific sequence characterized amplified region (SCAR) markers.

Keywords: AMOVA; shannons information index; species-specifc marker; UPGMA

Date published: 2017-09-26