Genetic diversity analysis of the Rhodope Shorthorn cattle breed based on SSR markers
Milena Bozhilova-Sakova, Denis Viryanski, Atanaska Teneva, Tsvetoslav Koynarski, Elena Todorovska, Ivona Dimitrova
Abstract: The aim of the present experiment was to study the genetic diversity in 79 individuals from the Rhodope Shorthorn cattle breed, raised in the region of Smolyan, Bulgaria. For the purpose of the experiment was used a panel of 11 microsatellite markers. A total of 103 alleles were identified and 7 of them were population-specific. The allele number per locus varied from 6 (BM1824) to 14 (TGLA53), with a mean number of 9.36. The highest number of heterozygotes (72) was observed in locus TGLA227 (n = 79). The lowest number of heterozygotes (41) was detected in locus ETH10S (n = 79). Microsatellite markers used in the current experiment showed PIC from 0.52 (ETH10) to 0.88 (ETH3), with a mean value of 0.897. Observed heterozygosity (Ho) varied from 0.767 (locus ETH10) to 0.946 (INRA23). Expected heterozygosity (genetic diversity - He) varied from 0.205 in locus ETH10 to 0.819 in locus TGLA227. Heterozygosity for all investigated loci was Ho = 0.897 and He = 0.586. Effective allele number (N) was between 7 (ETH10, INRA 23) and 14 (TGLA 53). Estimated values (PIC, Ho, He, and MNA) showed that all studied markers were polymorphic. All tested loci were with high Polymorphic information content (>0.5) and Ho > 0.6.
Keywords: Genetic diversity; Microsatellite markers; Polymorphism; Rhodope Shorthorn cattle
Citation: Bozhilova-Sakova, M., Viryanski, D., Teneva, A., Koynarski, Ts., Todorovska, E. & Dimitrova, I. (2023). Genetic diversity analysis of the Rhodope Shorthorn cattle breed based on SSR markers, Bulg. J. Agric. Sci., 29(6), 1143–1148.
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| Date published: 2023-12-15
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