Bulgarian Journal of Agricultural Science
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Comparative analysis of protein profile of Siberian sturgeon (Acipenser baerii), Russian sturgeon (Acipenser gueldenstaedtii), and hybrid (F1 Acipenser baerii x Acipenser gueldenstaedtii) grown on an aquaculture farm
Petya Ivanova, Vesela Chalova, Lyudmila Nikolova, Georgi Georgiev
Abstract: In the present study, an electrophoretic technique was used to determine protein profiles of sarcoplasmic, myofibrillar, and alkali-soluble proteins in sturgeon meat of different genotypes (Russian sturgeon, Siberian sturgeon and their F1 hybrid). SDSPAGE of the protein fractions showed that sarcoplasmic and myofibrillar proteins differed the most. High molecular weight polymers (>250 kDa) were observed in the myofibrillar fraction in each weight group of the three genotypes. The highest number of bands (20) was observed in the sarcoplasmic protein fraction of the Russian sturgeon. Low molecular weight fractions (<50 kDa) were dominant in each investigated genotype. In the studied hybrids, proteins with molecular weights which were not found in the studied individuals from the Russian and Siberian sturgeon, were registered. No such trend was observed in alkali-soluble proteins of the hybrids where all observed bands corresponded to the studied individuals Russian and Siberian sturgeon. The number of protein bands and their mobility (respectively molecular weight) is important for species characterization and identification.
Keywords: aquaculture; muscle proteins; sarcoplasmic, myofibrillar, alkali-soluble proteins; sturgeon
Date published: 2022-11-02
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