In vitro propagation of Vaccinium corymbosum L.
Maria Georgieva and Violeta Kondakova
Abstract: The production of Vaccinium sp. has been a growing worldwide interest after understending their importance for diet and human health.
The aim of the work was direct regeneration, propagation and rooting of axillary buds isolated from five highbush blueberry cultivars ‘Bluecrop’, ‘Bluegold’, ‘Bluejay’, ‘Spartan’ and ‘Patriot’ and adapt of suitable protocol, developed and applied for Vaccinium corymbosum L. cultivars in our laboratory.
For micropropagation, a basal cultural medium WPM was used, supplemented with 3.0 mg/L zeatin and 2.0 mg/l 2-iP, pH 4.2. The highest proliferation capacity was reported for cv. ‘Bluegold’ after fourth passage of sub cultivation (4.4 shoots per explant) and the largest length of the shoots was reported for the cv. ‘Bluejay’ (3.9 cm).
The rooting ability was established on the same basal medium (WPM) with half-reduced salt concentration enriched with 1.0 mg/L IAA, pH 4.2. The high rooting percentage (33.3%) and the highest average number of roots (3.9) was reported for cv. ‘Spartan’ and ‘Patriot’.
The results demonstrate that regeneration potential is genotyp dependent.
The aim of the work was direct regeneration, propagation and rooting of axillary buds isolated from five highbush blueberry cultivars ‘Bluecrop’, ‘Bluegold’, ‘Bluejay’, ‘Spartan’ and ‘Patriot’ and adapt of suitable protocol, developed and applied for Vaccinium corymbosum L. cultivars in our laboratory.
For micropropagation, a basal cultural medium WPM was used, supplemented with 3.0 mg/L zeatin and 2.0 mg/l 2-iP, pH 4.2. The highest proliferation capacity was reported for cv. ‘Bluegold’ after fourth passage of sub cultivation (4.4 shoots per explant) and the largest length of the shoots was reported for the cv. ‘Bluejay’ (3.9 cm).
The rooting ability was established on the same basal medium (WPM) with half-reduced salt concentration enriched with 1.0 mg/L IAA, pH 4.2. The high rooting percentage (33.3%) and the highest average number of roots (3.9) was reported for cv. ‘Spartan’ and ‘Patriot’.
The results demonstrate that regeneration potential is genotyp dependent.
Keywords: in vitro; micropropagation; rooting; Vaccinium corymbosum L.
Date published: 2021-04-16
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