ANTIBIORESISTANCE, PHYLOGENY AND VIRULENCE MARKERS OF ESCHERICHIA COLI STRAINS ISOLATED FROM CHICKEN MEAT COMMERCIALISED IN JIJEL AREA (EAST ALGERIA)

D. Bibbal, D. Boudjerda, M. Lehouel, H. Brugère

Abstract: This study was conducted to investigate the prevalence, phylogeny, virulence markers and antibioresistance of commensal Escherichia coli. In order to get this objective Eighty-five (85) Escherichia coli isolates were obtained from 100 chicken meat samples commercialised in butcheries localised in Jijel area (East Algeria). Our results showed that strains belonged to phylogenetic groups A and B1 were the most common 67.5% and 16.9% respectively followed by B2 4.7% and D 11.76% groups. Antibiogramme results revealed three great antibiotic groups and more than one hundred antibiotype, the first include antibiotics which have between 50% to 70% of resistant strains (Ampicillin, Amoxicillin, Trimethoprim, Sulphonamid, Tetracycline), the second one, have 20% to 50% of resistant strains (Amoxicillin plus Clavulanic acid, Kanamycin, Streptomycin, Ciprofloxacin, Flumequin, Neomycin) and the third group include less than 20% of resistant strains (Cephalothin, Gentamicin, Colistin). Furthermore, antibioresistance to Flumequin, Trimethoprim, Sulfamethoxazole-Trimethoprim, and Tetracycline are more frequent in analysed chicken meat samples. PCR investigation shown the predominance of strains with Extraintestinal pathogenic E. coli virulence factors related genes (2.4%) and the presence of strains with EAEC and EIEC genes. We noted also that strains carrying more than two virulence factors are belonged to B2 or D phylogenetic groups and present less resistance to antibiotics whereas strains belonged to group A or B1 present less virulence factors and high rate of antibioresistance.

Keywords: antibioresistance; chicken meat; Escherichia coli; phylogeny; virulence factors

Date published: 2017-08-30