Bulgarian Journal of Agricultural Science
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ANTIMICROBIAL SUSCEPTIBILITY OF STAPHYLOCOCCI ISOLATED FROM CLINICAL MASTITIS IN DAIRY COWS IN KOSOVO
A. Hamidi, D. Sylejmani
Abstract: This study was carried out to evaluate antibiotic susceptibility of 26 strains of coagulase negative staphylococci (CNS) and 18 strains of Staphylococcus aureus isolated from samples of dairy cows with clinical mastitis. The isolates species of staphylococci were identified using coagulase test (using rabbit plasma) and API Staph system (bioMėrieux SA) which differentiates S. aurues from other staphylococci. Isolates were tested for antimicrobial susceptibility on Mueller Hinton agar by dick diffusion method according to the Clinical Laboratory Standards Institute. The findings of this study showed that the most
effective in vitro antibiotics for S. aureus isolates, isolated from cows with clinical mastitis wereamoxycillin/clavulanic acid (83.3%), tetracycline (77.8%), gentamycin (77.8%) and trimethoprim (66.7%) and for CNS isolates trimethoprim (88.46%), amoxycillin (84.6%), tetracycline (77%) and gentamycin (69.2%) while the isolates of S. aureus and CNS were found to be more resistant to penicillin (55.5% and 53.8%, respectively), streptomycin (50% and 46.1%, respectively) and ampicillin (38.9% and 46.1%, respectively). Antimicrobial susceptibility tests are important for the selection of the most effective antimicrobial agent for treatment of bovine mastitis caused by staphylococci (Staph. aureus and CNS.Continous monitoring for antimicrobial susceptibility of Staphylococci isolated from dairy cows with clinical mastitis is recommended as an important component of efficiently and prudent antimicrobial use practices.
Keywords: antimicrobial susceptibility; bovine mastitis; Kosovo; staphylococci
Date published: 2017-08-28
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