Hybridization technique of black orchid (Coelogyne pandurata Lindley) to enrich the genetic diversity and to rescue the genetic extinction

Ahmad Yunus, Sri Hartati, Djati Waluyo Djoar, Nandariyah

Abstract: The existence of Coelogyne pandurata Lindley, an exotic and valuable orchid species from eastern Kalimantan is currently being endangered. The objectives of this study were to rescue the genetic extinction of the Coelogyne pandurata Lindley as well as to create new genetics through crossbreeding with other species. Crosses were performed at 4 to 6 flowering individuals. Pollens were transferred from the anther to the stigma by using sterile toothpicks, with following methods: crossing, reciprocal and self-pollination. The parameters observed included: percentage of success in crossing, percentage of fallen fruit, age of ripening fruit, time of protocorm formation.
The results showed that the abilities of crossing for each intersection were as follows: crossing (♀Coelogyne pandurata × ♂Coelogyne rumphii), reciprocal (♀Coelogyne rumphii × ♂Coelogyne pandurata) and selfing was 100%. The percentage of fallen fruit was as follows: crossing was 50%, reciprocal was 25% and self-pollination was 25%. Age of ripening fruit was as follows: crossing was 158 days, reciprocal was 191 days and selfing pollination was 155-201 days. Time of protocorm formation was as follows: crossing was 22 days, reciprocal was 48 days and selfi ng was 26-94 days.

Keywords: crossing; extinction; protocorm; reciprocal; self-pollination

Date published: 2019-08-27