Study on genetic diversity among Iranian water pipes tobacco (Nicotiana spp.) varieties by using simple sequence repeat markers

I. Bernousi, H. Hatami Maleki, R. Darvishzadeh, B. Abdollahi, S. Gholizadeh

Abstract: Water pipe’s tobacco is one of the most popular and important industrial plant cultivated in central parts of Iran. In this study, genetic variation among 13 Iranian water pipe’s tobacco (Nicotiana spp.) genotypes was assessed by using simple sequence repeat (SSR) markers. 100 alleles were generated at 30 SSR loci. The mean number of alleles per locus (na) and the effective allele number (ne) were 3.30 and 2.40, respectively. The expected heterozygosity ranged from 0.21 to 0.78 with average of 0.53. The Jaccard’s similarity coefficients among studied water pipe’s tobacco genotypes varied from 0.13 to 0.60 suggesting the presence of moderate genetic diversity among Iranian water pipe’s tobacco genotypes. Based on un-weighted pair group method using arithmetic average (UPGMA) clustering algorithm the studied water pipe’s tobacco genotypes were placed into 4 heterotic groups that could be applied in parent selection for water pipe’s tobacco breeding programs. Our clustering could distinct two species of water pipe’s tobacco including N. rustica and N. tabacum from each other successfully.

Keywords: cluster analysis; Genetic variability; heterotic groups; industrial plants; microsatellite markers

Date published: 2019-02-27