Immunochromatographic assay for the foot-and-mouth disease utilizing recombinant nonstructural proteins 2C, 3A, 3B and 3D

ZHANSAYA ADISH, KANAT TURSUNOV, ALEXANDER SHUSTOV, ERLAN RAMANKULOV, MALIKA AZHIBAYEVA, BAKHYTKALI INGIRBAY, KANATBEK MUKANTAYEV, KASYM MUKANOV, ZHULDYZ KAIROVA

Abstract: Foot-and-mouth disease (FMD) is a highly contagious infection of domestic and wild cloven-hoofed animals. Recommended measures to prevent transmission of the FMD from endemic areas is a vaccination of livestock in endangered territories and creation of a buffer zone in which susceptible animals are vaccinated. Because FMD vaccines do not provide long protective immunity control for possible infection in vaccinated animals is important in areas with vaccination. It is possible by using DIVA (Discrimination of Infected from Vaccinated Animals) assays which detect antibodies to non-structural proteins (NSPs) of the FMD virus. The paper describes development of an immunochromatographic assay (ICA) which uses recombinantly expressed NSPs 2C, 3A, 3B and 3D as antigens to capture specifi c antibodies. The recombinant FMD antigens were produced using expression in E. coli and purifi ed by metal affi nity column chromatography. Sensitivity and specifi city of the ICA were found to be 92-97% and 91-96%, respectively in samples of sera from animals with varying risk of the FMD.

Keywords: antigen; DIVA; foot-and-mouth disease; immunochromatographic assay; non-structural proteins; recombinant

Date published: 2019-04-09