Effects of media combination with concentration of AB-Mix nutrient on growth of banana shoots on in vitro

AHMAD YUNUS, SAMANHUDI, ENDANG YUNIASTUTI, HAFIDH PRABOWO

Abstract: This study aims to examine the effect of MS (Murashige and Skoog) media combination, coconut water (CW) and AB-Mix nutrition on the growth of banana shoots and to review growth differences in banana cultivars. The study was conducted from April-October 2017 at the Tissue Culture Laboratory of Balai Benih Hortikultura, Salaman, Magelang. The experimental design was designed using Completely Randomized Design (CRD) with two treatment factors. The first factor is media consisting of 5 levels and the second factor is cultivar consisting of 4 levels. Each combination is repeated 3 times as a sample. The results showed that the treatment of media and cultivars signifi cantly affected the growth of banana shoots in tissue culture for 42 days after planting (DAP). MS medium treatment showed the highest result on root number, root length, and plant vigor variables. The treatment of Mas Kirana banana cultivars showed the best results on the root number, root length, shoot length, and plant vigor variables.

Keywords: AB-Mix nutrition; banana; coconut water; phenol; tissue culture

Date published: 2018-06-12